Detailed information of TA system TA150166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 1810..2273
• Replicon: plasmid unnamed2
• Accession: NZ_CP048910
• Organism: Neisseria gonorrhoeae strain SRRSH203

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: Q51003
• Locus tag: G4V51_RS11895
• Protein ID: WP_003690225.1
• Coordinates: 1810..2088 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: Q51002
• Locus tag: G4V51_RS11900
• Protein ID: WP_003690223.1
• Coordinates: 2088..2273 (-)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4V51_RS11875	1..192	+	192	WP_003690218.1	plasmid mobilization relaxosome protein MobC	-
G4V51_RS11880	177..806	+	630	WP_003696452.1	relaxase/mobilization nuclease domain-containing protein	-
G4V51_RS11885	890..1531	+	642	WP_003690229.1	Cryptic plasmid protein B	-
G4V51_RS11890	1563..1832	+	270	WP_003692166.1	hypothetical protein	-
G4V51_RS11895	1810..2088	-	279	WP_003690225.1	endoribonuclease VapD	Toxin
G4V51_RS11900	2088..2273	-	186	WP_003690223.1	DUF5397 family protein	Antitoxin
G4V51_RS11905	2276..2521	-	246	WP_003690221.1	hypothetical protein	-
G4V51_RS11910	2518..2760	-	243	WP_003690220.1	HTH domain-containing protein	-
G4V51_RS11915	2764..3546	-	783	WP_010359605.1	replication initiation protein	-
G4V51_RS11920	3716..3940	-	225	WP_025455920.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..4207	4207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (2-60)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10473.89 Da
• Isoelectric Point: 6.9726

>T150166 WP_003690225.1 NZ_CP048910:c2088-1810 [Neisseria gonorrhoeae]
MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSLYTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRI
EQWSDFTSLVKS

Nucleotide

• Length: 279 bp

>T150166 NZ_CP064401:c179010-178729 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 6717.54 Da
• Isoelectric Point: 4.2641

>AT150166 WP_003690223.1 NZ_CP048910:c2273-2088 [Neisseria gonorrhoeae]
MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESGEHTQYKLSHIVQDPKAA

Nucleotide

• Length: 186 bp

>AT150166 NZ_CP064401:c179234-179007 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: A0A7H5FJI5

Antitoxin

• AlphaFold DB: Q51002