Detailed information of TA system TA150165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta(toxin)
• Location: 10356..12007
• Replicon: plasmid unnamed1
• Accession: NZ_CP048909
• Organism: Neisseria gonorrhoeae strain SRRSH203

Toxin (Protein)

• Gene name: ng_zeta1
• Uniprot ID: -
• Locus tag: G4V51_RS11715
• Protein ID: WP_033911112.1
• Coordinates: 10356..11561 (+)
• Length: 402 a.a.

Antitoxin (Protein)

• Gene name: ng_epsilon1
• Uniprot ID: D5K9G8
• Locus tag: G4V51_RS11720
• Protein ID: WP_003701903.1
• Coordinates: 11576..12007 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4V51_RS11680	5944..6312	+	369	WP_003701920.1	hypothetical protein	-
G4V51_RS11685	6296..6667	+	372	WP_003701919.1	hypothetical protein	-
G4V51_RS11690	6671..7567	+	897	WP_003701917.1	hypothetical protein	-
G4V51_RS11695	7719..8207	-	489	WP_014580055.1	hypothetical protein	-
G4V51_RS11700	8677..8925	+	249	WP_014580056.1	hypothetical protein	-
G4V51_RS11705	8922..10130	+	1209	WP_014580057.1	zeta toxin family protein	-
G4V51_RS11710	10164..10349	+	186	WP_014580058.1	hypothetical protein	-
G4V51_RS11715	10356..11561	+	1206	WP_033911112.1	zeta toxin family protein	Toxin
G4V51_RS11720	11576..12007	+	432	WP_003701903.1	hypothetical protein	Antitoxin
G4V51_RS11725	12059..12613	+	555	WP_003701901.1	recombinase family protein	-
G4V51_RS11730	12723..13013	-	291	WP_003701899.1	virulence factor	-
G4V51_RS11735	13116..13715	-	600	WP_003701898.1	TrbN protein	-
G4V51_RS11740	13715..14293	-	579	WP_033911116.1	TrbM protein	-
G4V51_RS11745	14280..15907	-	1628	Protein_19	P-type conjugative transfer protein TrbL	-
G4V51_RS11750	16121..16897	-	777	WP_003701891.1	P-type conjugative transfer protein TrbJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39059	39059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• KfrB domain (284-343)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 402 a.a.
• Molecular weight: 44747.59 Da
• Isoelectric Point: 7.2930

>T150165 WP_033911112.1 NZ_CP048909:10356-11561 [Neisseria gonorrhoeae]
MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQHADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHN
SAAVLSSTQFDEVLKRDEPQKKEVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
VSMRAERASDNTYKRFNEYGREASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQDRNSEFIDKFDDVIKMLSLGSQE
QILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESVFAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGI
YLLGQAKKAETGQTYSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLEYSAKKSIS
R

Nucleotide

• Length: 1206 bp

>T150165 NZ_CP064399:c5521876-5521598 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCTTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15927.05 Da
• Isoelectric Point: 4.4211

>AT150165 WP_003701903.1 NZ_CP048909:11576-12007 [Neisseria gonorrhoeae]
MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLADILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVR
LSPFLNGCPHDLSLGAESLGKGESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ

Nucleotide

• Length: 432 bp

>AT150165 NZ_CP064399:c5521586-5521281 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Antitoxin

• AlphaFold DB: D5K9G8