Detailed information of TA system TA150162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta(toxin)
• Location: 3267..4918
• Replicon: plasmid unnamed1
• Accession: NZ_CP048906
• Organism: Neisseria gonorrhoeae strain SRRSH205

Toxin (Protein)

• Gene name: ng_zeta1
• Uniprot ID: -
• Locus tag: G4V52_RS11670
• Protein ID: WP_033911112.1
• Coordinates: 3713..4918 (-)
• Length: 402 a.a.

Antitoxin (Protein)

• Gene name: ng_epsilon1
• Uniprot ID: D5K9G8
• Locus tag: G4V52_RS11665
• Protein ID: WP_003701903.1
• Coordinates: 3267..3698 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4V52_RS11645	981..1559	+	579	WP_033911116.1	TrbM protein	-
G4V52_RS11650	1559..2158	+	600	WP_003701898.1	TrbN protein	-
G4V52_RS11655	2261..2551	+	291	WP_003701899.1	virulence factor	-
G4V52_RS11660	2661..3215	-	555	WP_003701901.1	recombinase family protein	-
G4V52_RS11665	3267..3698	-	432	WP_003701903.1	hypothetical protein	Antitoxin
G4V52_RS11670	3713..4918	-	1206	WP_033911112.1	zeta toxin family protein	Toxin
G4V52_RS11675	4925..5110	-	186	WP_014580058.1	hypothetical protein	-
G4V52_RS11680	5144..6352	-	1209	WP_014580057.1	zeta toxin family protein	-
G4V52_RS11685	6349..6597	-	249	WP_014580056.1	hypothetical protein	-
G4V52_RS11690	7067..7555	+	489	WP_014580055.1	hypothetical protein	-
G4V52_RS11695	7707..8603	-	897	WP_003701917.1	hypothetical protein	-
G4V52_RS11700	8607..8978	-	372	WP_003701919.1	hypothetical protein	-
G4V52_RS11705	8962..9330	-	369	WP_003701920.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39059	39059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• KfrB domain (284-343)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 402 a.a.
• Molecular weight: 44747.59 Da
• Isoelectric Point: 7.2930

>T150162 WP_033911112.1 NZ_CP048906:c4918-3713 [Neisseria gonorrhoeae]
MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQHADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHN
SAAVLSSTQFDEVLKRDEPQKKEVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
VSMRAERASDNTYKRFNEYGREASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQDRNSEFIDKFDDVIKMLSLGSQE
QILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESVFAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGI
YLLGQAKKAETGQTYSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLEYSAKKSIS
R

Nucleotide

• Length: 1206 bp

>T150162 NZ_CP064399:c4531198-4530788 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCGGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACT
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGAAAACAAATCGGCCCCTATGACCAGATGATCGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTTGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCGCCC
CAATCGTGTAG

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15927.05 Da
• Isoelectric Point: 4.4211

>AT150162 WP_003701903.1 NZ_CP048906:c3698-3267 [Neisseria gonorrhoeae]
MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLADILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVR
LSPFLNGCPHDLSLGAESLGKGESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ

Nucleotide

• Length: 432 bp

>AT150162 NZ_CP064399:c4531428-4531198 [Pseudomonas aeruginosa]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTTCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: D5K9G8