Detailed information of TA system TA150160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 1710..2173
• Replicon: plasmid unnamed2
• Accession: NZ_CP048904
• Organism: Neisseria gonorrhoeae strain SRRSH207

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: Q51003
• Locus tag: G4V53_RS11840
• Protein ID: WP_003690225.1
• Coordinates: 1895..2173 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: Q51002
• Locus tag: G4V53_RS11835
• Protein ID: WP_003690223.1
• Coordinates: 1710..1895 (+)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4V53_RS11815	43..267	+	225	WP_025455920.1	hypothetical protein	-
G4V53_RS11820	437..1219	+	783	WP_010359605.1	replication initiation protein	-
G4V53_RS11825	1223..1465	+	243	WP_003690220.1	HTH domain-containing protein	-
G4V53_RS11830	1462..1707	+	246	WP_003690221.1	hypothetical protein	-
G4V53_RS11835	1710..1895	+	186	WP_003690223.1	DUF5397 family protein	Antitoxin
G4V53_RS11840	1895..2173	+	279	WP_003690225.1	endoribonuclease VapD	Toxin
G4V53_RS11845	2151..2420	-	270	WP_003692166.1	hypothetical protein	-
G4V53_RS11850	2452..3093	-	642	WP_003690229.1	Cryptic plasmid protein B	-
G4V53_RS11855	3177..3806	-	630	WP_003696452.1	relaxase/mobilization nuclease domain-containing protein	-
G4V53_RS11860	3791..3982	-	192	WP_003690218.1	plasmid mobilization relaxosome protein MobC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..4207	4207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (2-60)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10473.89 Da
• Isoelectric Point: 6.9726

>T150160 WP_003690225.1 NZ_CP048904:1895-2173 [Neisseria gonorrhoeae]
MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSLYTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRI
EQWSDFTSLVKS

Nucleotide

• Length: 279 bp

>T150160 NZ_CP064399:3522319-3522897 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
TCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGTGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 6717.54 Da
• Isoelectric Point: 4.2641

>AT150160 WP_003690223.1 NZ_CP048904:1710-1895 [Neisseria gonorrhoeae]
MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESGEHTQYKLSHIVQDPKAA

Nucleotide

• Length: 186 bp

>AT150160 NZ_CP064399:3521676-3522230 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Structures

Toxin

• AlphaFold DB: A0A7H5FJI5

Antitoxin

• AlphaFold DB: Q51002