Detailed information of TA system TA150157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 12..4034
• Replicon: plasmid unnamed2
• Accession: NZ_CP048901
• Organism: Neisseria gonorrhoeae strain SRRSH214

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: Q51003
• Locus tag: G4V50_RS11840
• Protein ID: WP_003690225.1
• Coordinates: 12..290 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: Q51002
• Locus tag: G4V50_RS11835
• Protein ID: WP_003690223.1
• Coordinates: 4034..12 (+)
• Length: -1340.3333333333 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4V50_RS11840	12..290	+	279	WP_003690225.1	endoribonuclease VapD	Toxin
G4V50_RS11845	268..522	-	255	WP_003690227.1	Cryptic plasmid protein A	-
G4V50_RS11850	569..1210	-	642	WP_003690229.1	Cryptic plasmid protein B	-
G4V50_RS11855	1294..1923	-	630	WP_003696452.1	relaxase/mobilization nuclease domain-containing protein	-
G4V50_RS11860	1908..2099	-	192	WP_003690218.1	plasmid mobilization relaxosome protein MobC	-
G4V50_RS11865	2367..2591	+	225	WP_025455920.1	hypothetical protein	-
G4V50_RS11870	2761..3543	+	783	WP_010359605.1	replication initiation protein	-
G4V50_RS11875	3547..3789	+	243	WP_003690220.1	HTH domain-containing protein	-
G4V50_RS11880	3786..4031	+	246	WP_003690221.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..4207	4207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10473.89 Da
• Isoelectric Point: 6.9726

>T150157 WP_003690225.1 NZ_CP048901:12-290 [Neisseria gonorrhoeae]
MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSLYTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRI
EQWSDFTSLVKS

Nucleotide

• Length: 279 bp

>T150157 NZ_CP064397:c5871733-5871455 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTTGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: -1340.3333333333 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT150157 WP_003690223.1 NZ_CP048901:4034-12 [Neisseria gonorrhoeae]

Nucleotide

• Length: -4021 bp

>AT150157 NZ_CP064397:c5871443-5871138 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A7H5FJI5

Antitoxin

• AlphaFold DB: Q51002