Detailed information of TA system TA150156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta(toxin)
• Location: 9725..11376
• Replicon: plasmid unnamed1
• Accession: NZ_CP048900
• Organism: Neisseria gonorrhoeae strain SRRSH214

Toxin (Protein)

• Gene name: ng_zeta1
• Uniprot ID: -
• Locus tag: G4V50_RS11675
• Protein ID: WP_033911112.1
• Coordinates: 9725..10930 (+)
• Length: 402 a.a.

Antitoxin (Protein)

• Gene name: ng_epsilon1
• Uniprot ID: D5K9G8
• Locus tag: G4V50_RS11680
• Protein ID: WP_003701903.1
• Coordinates: 10945..11376 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4V50_RS11640	5313..5681	+	369	WP_003701920.1	hypothetical protein	-
G4V50_RS11645	5665..6036	+	372	WP_003701919.1	hypothetical protein	-
G4V50_RS11650	6040..6936	+	897	WP_003701917.1	hypothetical protein	-
G4V50_RS11655	7088..7576	-	489	WP_014580055.1	hypothetical protein	-
G4V50_RS11660	8046..8294	+	249	WP_014580056.1	hypothetical protein	-
G4V50_RS11665	8291..9499	+	1209	WP_014580057.1	zeta toxin family protein	-
G4V50_RS11670	9533..9718	+	186	WP_014580058.1	hypothetical protein	-
G4V50_RS11675	9725..10930	+	1206	WP_033911112.1	zeta toxin family protein	Toxin
G4V50_RS11680	10945..11376	+	432	WP_003701903.1	hypothetical protein	Antitoxin
G4V50_RS11685	11428..11982	+	555	WP_003701901.1	recombinase family protein	-
G4V50_RS11690	12092..12382	-	291	WP_003701899.1	virulence factor	-
G4V50_RS11695	12485..13084	-	600	WP_003701898.1	TrbN protein	-
G4V50_RS11700	13084..13662	-	579	WP_033911116.1	TrbM protein	-
G4V50_RS11705	13649..15276	-	1628	Protein_19	P-type conjugative transfer protein TrbL	-
G4V50_RS11710	15490..16266	-	777	WP_003701891.1	P-type conjugative transfer protein TrbJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..39059	39059

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• KfrB domain (284-343)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 402 a.a.
• Molecular weight: 44747.59 Da
• Isoelectric Point: 7.2930

>T150156 WP_033911112.1 NZ_CP048900:9725-10930 [Neisseria gonorrhoeae]
MVKLSSDINLRDFGNNEYLSSVQDEAIRFATEQTDEILSLYSQHADTEGGRYVCADTFKELFPAFENKEDRATVNNAIHN
SAAVLSSTQFDEVLKRDEPQKKEVIFVTGIPGSGKTSTVKNMMMQDTTKLLFEGQLARPQSAFRKIEQCLERNLEVTIVA
VSMRAERASDNTYKRFNEYGREASIGIMADIQANLPDGLKQIRDKFGDAVKIVGINQDRNSEFIDKFDDVIKMLSLGSQE
QILGRLAEKIQSDFDSGKISRECFNQAKGSMDLESVFAKKEYSQQRVVTNSKGVTLETKSANELWSKVEQIPVTGMKAGI
YLLGQAKKAETGQTYSGEIIYKDAAAVFQKTKNGLVRHNATHNEERLAKLVEIGQNVSIGSNKGKLIVKSLEYSAKKSIS
R

Nucleotide

• Length: 1206 bp

>T150156 NZ_CP064397:c5653901-5653602 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15927.05 Da
• Isoelectric Point: 4.4211

>AT150156 WP_003701903.1 NZ_CP048900:10945-11376 [Neisseria gonorrhoeae]
MKEKMRLITEISLIIRELSYSRRGYRPESYAAALDNLTIIGDLADILHNIEAATGNPFVQRMVDEKLRKFVTDFPAYKVR
LSPFLNGCPHDLSLGAESLGKGESIADALAVPPEEVDIADIEFDRVLIEDDRWTGAYDGGNAQ

Nucleotide

• Length: 432 bp

>AT150156 NZ_CP064397:c5653605-5653315 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Antitoxin

• AlphaFold DB: D5K9G8