Detailed information of TA system TA150152

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 508..971
• Replicon: plasmid unnamed2
• Accession: NZ_CP048895
• Organism: Neisseria gonorrhoeae strain SRRSH240

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: Q51003
• Locus tag: G4V55_RS11840
• Protein ID: WP_003690225.1
• Coordinates: 508..786 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: Q51002
• Locus tag: G4V55_RS11845
• Protein ID: WP_003690223.1
• Coordinates: 786..971 (-)
• Length: 62 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4V55_RS11835	276..530	+	255	WP_003690227.1	Cryptic plasmid protein A	-
G4V55_RS11840	508..786	-	279	WP_003690225.1	endoribonuclease VapD	Toxin
G4V55_RS11845	786..971	-	186	WP_003690223.1	DUF5397 family protein	Antitoxin
G4V55_RS11850	974..1219	-	246	WP_003690221.1	hypothetical protein	-
G4V55_RS11855	1216..1458	-	243	WP_003690220.1	HTH domain-containing protein	-
G4V55_RS11860	1462..2244	-	783	WP_010359605.1	replication initiation protein	-
G4V55_RS11865	2414..2638	-	225	WP_025455920.1	hypothetical protein	-
G4V55_RS11870	2906..3097	+	192	WP_003690218.1	plasmid mobilization relaxosome protein MobC	-
G4V55_RS11875	3082..3711	+	630	WP_003696452.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..4207	4207

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (2-60)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10473.89 Da
• Isoelectric Point: 6.9726

>T150152 WP_003690225.1 NZ_CP048895:c786-508 [Neisseria gonorrhoeae]
MYAISFDLVVADTAQNHPKGISQAYADIGYTLRKFGFTRIQGSLYTCQNEDMANLFSAINELKALPWFPSSVRDIRAFRI
EQWSDFTSLVKS

Nucleotide

• Length: 279 bp

>T150152 NZ_CP064397:3718091-3718669 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGTGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin

Protein

• Length: 62 a.a.
• Molecular weight: 6717.54 Da
• Isoelectric Point: 4.2641

>AT150152 WP_003690223.1 NZ_CP048895:c971-786 [Neisseria gonorrhoeae]
MQTTIPTGSIRTFGDYGVMYIVGTPAEQLPDGDWLVNIELPESGEHTQYKLSHIVQDPKAA

Nucleotide

• Length: 186 bp

>AT150152 NZ_CP064397:3717449-3718003 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Structures

Toxin

• AlphaFold DB: A0A7H5FJI5

Antitoxin

• AlphaFold DB: Q51002