Detailed information of TA system TA150148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 29303..29939
• Replicon: chromosome
• Accession: NZ_CP048883
• Organism: Priestia megaterium strain Mes11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D5DWT4
• Locus tag: BMG_RS00215
• Protein ID: WP_013055004.1
• Coordinates: 29303..29653 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: D5DWT3
• Locus tag: BMG_RS00220
• Protein ID: WP_013055003.1
• Coordinates: 29658..29939 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BMG_RS00180	24887..25681	-	795	WP_013055011.1	RNA polymerase sigma factor SigB	-
BMG_RS00185	25647..26132	-	486	WP_013055010.1	anti-sigma B factor RsbW	-
BMG_RS00190	26129..26461	-	333	WP_013055009.1	anti-sigma factor antagonist	-
BMG_RS00195	26521..27531	-	1011	WP_013055008.1	PP2C family protein-serine/threonine phosphatase	-
BMG_RS00200	27545..27946	-	402	WP_013055007.1	anti-sigma regulatory factor	-
BMG_RS00205	27950..28306	-	357	WP_013055006.1	STAS domain-containing protein	-
BMG_RS00210	28309..29145	-	837	WP_013055005.1	STAS domain-containing protein	-
BMG_RS00215	29303..29653	-	351	WP_013055004.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BMG_RS00220	29658..29939	-	282	WP_013055003.1	hypothetical protein	Antitoxin
BMG_RS00225	30140..31330	-	1191	WP_013055002.1	alanine racemase	-
BMG_RS00230	31444..32502	-	1059	WP_013055001.1	outer membrane lipoprotein carrier protein LolA	-
BMG_RS00235	32561..32926	-	366	WP_013055000.1	holo-ACP synthase	-
BMG_RS00240	32991..33593	+	603	WP_013054999.1	rhomboid family intramembrane serine protease	-
BMG_RS00245	33602..34573	-	972	WP_035439664.1	UV DNA damage repair endonuclease UvsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12991.96 Da
• Isoelectric Point: 4.8781

>T150148 WP_013055004.1 NZ_CP048883:c29653-29303 [Priestia megaterium]
MVVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDRVDEALQISVGLIDF

Nucleotide

• Length: 351 bp

>T150148 NZ_CP064395:c5858595-5858317 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTTGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.22 Da
• Isoelectric Point: 8.5339

>AT150148 WP_013055003.1 NZ_CP048883:c29939-29658 [Priestia megaterium]
VSEASATTEILVRLPKNLVSELDLMVKQENGNRSDFIHQATKLYLRERKKRHIRESMRRGYMEMAKINLNIASEAFLAEY
EADHTVDRLVSGG

Nucleotide

• Length: 282 bp

>AT150148 NZ_CP064395:c5858305-5858000 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Antitoxin

• AlphaFold DB: A0A0B6AMB9