Detailed information of TA system TA150140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 282..920
• Replicon: plasmid pB
• Accession: NZ_CP048880
• Organism: Spartinivicinus ruber strain S2-4-1H

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G4Y78_RS30695
• Protein ID: WP_178124488.1
• Coordinates: 282..683 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: -
• Locus tag: G4Y78_RS30700
• Protein ID: WP_178124489.1
• Coordinates: 684..920 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4Y78_RS30695	282..683	-	402	WP_178124488.1	type II toxin-antitoxin system VapC family toxin	Toxin
G4Y78_RS30700	684..920	-	237	WP_178124489.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G4Y78_RS29430	1206..1385	+	180	WP_163836801.1	hypothetical protein	-
G4Y78_RS29435	1355..2266	-	912	WP_163836802.1	transcriptional activator NhaR	-
G4Y78_RS29440	2466..2813	+	348	WP_163836803.1	HPF/RaiA family ribosome-associated protein	-
G4Y78_RS29445	2860..3522	+	663	WP_163836804.1	Bax inhibitor-1/YccA family protein	-
G4Y78_RS29450	3734..3928	+	195	WP_163836805.1	type II toxin-antitoxin system VapB family antitoxin	-
G4Y78_RS29455	3928..4308	+	381	WP_163836806.1	VapC toxin family PIN domain ribonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..102423	102423

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-120)

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14932.24 Da
• Isoelectric Point: 6.2320

>T150140 WP_178124488.1 NZ_CP048880:c683-282 [Spartinivicinus ruber]
MKYMLDTNICIYLMKKHPKEVADRFADCYYGDVVISSITLGELEYGVEVSAEKERNAQALKLLLEDIPVMPFEDKAAQSY
GVLRAGAPKRKGDALDKLIAAHAKSLDVVLVTNNVKDFQKYPGITIENWVNNH

Nucleotide

• Length: 402 bp

>T150140 NZ_CP064395:c176383-176102 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9185.41 Da
• Isoelectric Point: 4.6838

>AT150140 WP_178124489.1 NZ_CP048880:c920-684 [Spartinivicinus ruber]
MSLTKVFKSGNSQAVRIPAELQFDRLDMDYEIERIGDELRIRPAKRRLTNLMKKFEGFSDDFMAQGRESDDEPERDML

Nucleotide

• Length: 237 bp

>AT150140 NZ_CP064395:c176607-176380 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA