Detailed information of TA system TA150116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1265655..1266571
• Replicon: chromosome
• Accession: NZ_CP048852
• Organism: Bacillus tequilensis strain EA-CB0015

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6H0WG41
• Locus tag: G4P54_RS06785
• Protein ID: WP_024716366.1
• Coordinates: 1265825..1266571 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: A0A6H0WG39
• Locus tag: G4P54_RS06780
• Protein ID: WP_024716367.1
• Coordinates: 1265655..1265825 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4P54_RS06745	1262105..1262545	+	441	WP_167872171.1	hypothetical protein	-
G4P54_RS06750	1262535..1262726	+	192	WP_167872172.1	XkdX family protein	-
G4P54_RS06755	1262789..1263067	+	279	WP_167872173.1	hemolysin XhlA family protein	-
G4P54_RS06760	1263080..1263343	+	264	WP_167872174.1	phage holin	-
G4P54_RS06765	1263356..1264252	+	897	WP_167872175.1	N-acetylmuramoyl-L-alanine amidase	-
G4P54_RS06770	1264329..1265420	+	1092	WP_167872176.1	acyltransferase family protein	-
G4P54_RS06775	1265417..1265569	-	153	WP_167872177.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
G4P54_RS06780	1265655..1265825	-	171	WP_024716367.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
G4P54_RS06785	1265825..1266571	-	747	WP_024716366.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
G4P54_RS06790	1266682..1267683	-	1002	WP_024716365.1	inorganic phosphate transporter	-
G4P54_RS06795	1267696..1268313	-	618	WP_024716364.1	DUF47 domain-containing protein	-
G4P54_RS06800	1268591..1269907	-	1317	WP_024716363.1	serine/threonine exchanger	-
G4P54_RS06805	1270296..1271246	+	951	WP_024716362.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29036.52 Da
• Isoelectric Point: 4.4263

>T150116 WP_024716366.1 NZ_CP048852:c1266571-1265825 [Bacillus tequilensis]
MLLFFQIMVWCIMAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMIYWTYEPSSLFADWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CPYSTQADKDRLTEHMDDPTDVQTRLDRKDVYYDQYGKMVLIPFTIDTQSYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T150116 NZ_CP064389:c2582437-2582192 [Staphylococcus aureus]
ATGAACATTCAAGAAGCAACTAAGATAGCTACAAAAAATCTTGTCTCTATGACACGGAAAGATTGGAAAGAAAGTCATCG
AACTAAGATATTACCAACAAATGATAGTTTTTTACAATGCATCATTTCAAATAGCGATGGGACAAACCTTATCAGATATT
GGCAACCTTCAGCCGATGACCTCATGGCAAATGATTGGGAAGTTATAAACCCAACTAGAGACCAGGAATTATTGAAGCAA
TTTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6469.50 Da
• Isoelectric Point: 11.0264

>AT150116 WP_024716367.1 NZ_CP048852:c1265825-1265655 [Bacillus tequilensis]
MDRAFQNGCGPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT150116 NZ_CP064389:2581858-2582223 [Staphylococcus aureus]
ATGCCCTTGATATCTGATGAATTTGATACACTTACTAAAGACCAACAATATATCTTGTCCGTACTCTACAAAGATTATTT
AGAATGTGTAAAGTTAGGTTCGGTTAAATTAACCTGCAATAATTTTGGAAGTGCTAAAGATATACATACAAAGTATTTTC
AAAAACTACATTTCGAAGATGTAAAATACGATTTAAATAAACTTAAAAACTCTGGGTTCCTAAACGGCGTGTATGCTAGT
AACACTATTTATCATGTAACAATTTCAGACAAGACTGTTGTTTACTTTGAAAATGAGTTTAAAAACAATTTAAAAAGTAT
CATTGATAGCATTTCTAAAATTGCTTCAATAATTCCTGGTCTCTAG

Structures

Toxin

• AlphaFold DB: A0A6H0WG41

Antitoxin

• AlphaFold DB: A0A6H0WG39