Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 495817..496453 Replicon chromosome
Accession NZ_CP048852
Organism Bacillus tequilensis strain EA-CB0015

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag G4P54_RS02550 Protein ID WP_003156187.1
Coordinates 496103..496453 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag G4P54_RS02545 Protein ID WP_003225183.1
Coordinates 495817..496098 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4P54_RS02525 492179..492778 - 600 WP_024714452.1 rhomboid family intramembrane serine protease -
G4P54_RS02530 492874..493239 + 366 WP_167871688.1 holo-ACP synthase -
G4P54_RS02535 493404..494420 + 1017 WP_081719823.1 outer membrane lipoprotein carrier protein LolA -
G4P54_RS02540 494533..495702 + 1170 WP_167873807.1 alanine racemase -
G4P54_RS02545 495817..496098 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
G4P54_RS02550 496103..496453 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
G4P54_RS02555 496568..497392 + 825 WP_081719822.1 RsbT co-antagonist protein RsbRA -
G4P54_RS02560 497397..497762 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
G4P54_RS02565 497766..498167 + 402 WP_024714447.1 serine/threonine-protein kinase RsbT -
G4P54_RS02570 498179..499186 + 1008 WP_024714446.1 phosphoserine phosphatase RsbU -
G4P54_RS02575 499248..499577 + 330 WP_024714445.1 anti-sigma factor antagonist RsbV -
G4P54_RS02580 499574..500056 + 483 WP_024714444.1 anti-sigma B factor RsbW -
G4P54_RS02585 500022..500810 + 789 WP_024714443.1 RNA polymerase sigma factor SigB -
G4P54_RS02590 500810..501409 + 600 WP_024714442.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T150114 WP_003156187.1 NZ_CP048852:496103-496453 [Bacillus tequilensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T150114 NZ_CP064389:2006910-2007098 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTAGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTATCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT150114 WP_003225183.1 NZ_CP048852:495817-496098 [Bacillus tequilensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT150114 NZ_CP064389:2006621-2006881 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References