Detailed information of TA system TA150103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 2268492..2269088
• Replicon: chromosome
• Accession: NZ_CP048833
• Organism: Pseudomonas multiresinivorans strain populi

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: G4G71_RS10495
• Protein ID: WP_169937389.1
• Coordinates: 2268747..2269088 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A6G6J1L4
• Locus tag: G4G71_RS10490
• Protein ID: WP_024763399.1
• Coordinates: 2268492..2268740 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4G71_RS10465	2263508..2263759	+	252	WP_169937381.1	major capsid protein	-
G4G71_RS10470	2263906..2265225	+	1320	WP_169937383.1	attachment protein	-
G4G71_RS10475	2265231..2265587	+	357	WP_084358758.1	DUF2523 domain-containing protein	-
G4G71_RS10480	2265591..2266877	+	1287	WP_169937385.1	hypothetical protein	-
G4G71_RS10485	2267117..2268391	+	1275	WP_169937387.1	hypothetical protein	-
G4G71_RS10490	2268492..2268740	+	249	WP_024763399.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
G4G71_RS10495	2268747..2269088	+	342	WP_169937389.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G4G71_RS10500	2269440..2270312	-	873	WP_169937391.1	methyltransferase domain-containing protein	-
G4G71_RS10505	2270537..2270905	-	369	WP_169942605.1	hypothetical protein	-
G4G71_RS10510	2271004..2271351	-	348	WP_169937394.1	carboxymuconolactone decarboxylase family protein	-
G4G71_RS10515	2271485..2271967	-	483	WP_169937396.1	GreA/GreB family elongation factor	-
G4G71_RS10520	2272092..2272526	+	435	WP_169937398.1	hypothetical protein	-
G4G71_RS10525	2272533..2272991	-	459	WP_169937400.1	RcnB family protein	-
G4G71_RS10530	2273261..2273629	+	369	WP_024763405.1	DUF2384 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12976.64 Da
• Isoelectric Point: 4.5106

>T150103 WP_169937389.1 NZ_CP048833:2268747-2269088 [Pseudomonas multiresinivorans]
MTAREIRFTETAVFSIQDQEEHLAEYHPPELAAAKIDGLIDEILTRLQDAPVGYPVSRQASDLGVTRYRELNHDGYRVFY
EVYEHDNVIAVELVLRQKQDVEAALIRYCLVGI

Nucleotide

• Length: 342 bp

>T150103 NZ_CP064388:c26155-25880 [Escherichia coli O104:H4]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9312.72 Da
• Isoelectric Point: 9.5977

>AT150103 WP_024763399.1 NZ_CP048833:2268492-2268740 [Pseudomonas multiresinivorans]
MRVETISYLKRHAADLDLDEPMVVTQNGVPTLVVESYAERKRRDQAIALVKLLAMGSREYAEGKHCSADELKARLAKRRS
QQ

Nucleotide

• Length: 249 bp

>AT150103 NZ_CP064388:c26439-26155 [Escherichia coli O104:H4]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A6G6J1L4