Detailed information of TA system TA150100

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 510559..511064
• Replicon: chromosome
• Accession: NZ_CP048833
• Organism: Pseudomonas multiresinivorans strain populi

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: G4G71_RS02390
• Protein ID: WP_169935258.1
• Coordinates: 510783..511064 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: G4G71_RS02385
• Protein ID: WP_169935257.1
• Coordinates: 510559..510786 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4G71_RS02360	505821..506222	-	402	WP_169935252.1	GFA family protein	-
G4G71_RS02365	506238..506951	-	714	WP_169935253.1	phytanoyl-CoA dioxygenase family protein	-
G4G71_RS02370	506948..507874	-	927	WP_169935254.1	LysR family transcriptional regulator	-
G4G71_RS02375	507950..508906	-	957	WP_169935255.1	agmatinase	-
G4G71_RS02380	508933..510318	-	1386	WP_169935256.1	sodium:solute symporter	-
G4G71_RS02385	510559..510786	+	228	WP_169935257.1	CopG family ribbon-helix-helix protein	Antitoxin
G4G71_RS02390	510783..511064	+	282	WP_169935258.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G4G71_RS02395	511309..511596	-	288	WP_169935259.1	DUF2218 domain-containing protein	-
G4G71_RS02400	511694..512611	+	918	WP_169935260.1	LysR family transcriptional regulator	-
G4G71_RS02405	512655..513110	+	456	WP_169935261.1	GNAT family N-acetyltransferase	-
G4G71_RS02410	513112..514119	-	1008	WP_169942479.1	AraC family transcriptional regulator	-
G4G71_RS02415	514214..515164	-	951	WP_169935262.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10309.73 Da
• Isoelectric Point: 5.0336

>T150100 WP_169935258.1 NZ_CP048833:510783-511064 [Pseudomonas multiresinivorans]
MSLQWTYKAAADLDAIYDHYVVLIGPEKALRAIQDIVEQVSPLANLDLSSSGVPSEVPGVRELGVERWPYQAAFRVKGRS
VQILRIDRVDNSG

Nucleotide

• Length: 282 bp

>T150100 NZ_CP064387:5054375-5054773 [Escherichia coli O104:H4]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8259.21 Da
• Isoelectric Point: 5.9478

>AT150100 WP_169935257.1 NZ_CP048833:510559-510786 [Pseudomonas multiresinivorans]
VTTVVSFRADDSLVAALDQLARATHRDRPYHLRQALAQYLERQSWHVAAIDEGVADADAGHLLEHAAIEAKWGLA

Nucleotide

• Length: 228 bp

>AT150100 NZ_CP064387:5054148-5054375 [Escherichia coli O104:H4]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA