Detailed information of TA system TA150099

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 8142..8782
• Replicon: chromosome
• Accession: NZ_CP048833
• Organism: Pseudomonas multiresinivorans strain populi

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: G4G71_RS00030
• Protein ID: WP_169934904.1
• Coordinates: 8576..8782 (-)
• Length: 69 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: G4G71_RS00025
• Protein ID: WP_169934903.1
• Coordinates: 8142..8546 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4G71_RS00015	4257..7991	+	3735	WP_169934902.1	bifunctional diguanylate cyclase/phosphodiesterase	-
G4G71_RS00025	8142..8546	-	405	WP_169934903.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
G4G71_RS00030	8576..8782	-	207	WP_169934904.1	type II toxin-antitoxin system HicA family toxin	Toxin
G4G71_RS00035	9221..9706	+	486	WP_169934905.1	Bro-N domain-containing protein	-
G4G71_RS00040	10147..10470	+	324	WP_169934906.1	hypothetical protein	-
G4G71_RS00045	10635..12815	+	2181	WP_169934907.1	EAL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	162..12815	12653

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7409.56 Da
• Isoelectric Point: 10.4016

>T150099 WP_169934904.1 NZ_CP048833:c8782-8576 [Pseudomonas multiresinivorans]
MRSKEIIDLLIEDGWFEIGCNGSHHQFKHPVRPGKVTVPHPKASLPKGTVNSILKQAGLKLGQSSASH

Nucleotide

• Length: 207 bp

>T150099 NZ_CP064387:4745865-4746200 [Escherichia coli O104:H4]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14630.33 Da
• Isoelectric Point: 4.3897

>AT150099 WP_169934903.1 NZ_CP048833:c8546-8142 [Pseudomonas multiresinivorans]
MKFPVVLHKDPDSDYGVTLPDVSGCFSAGCTVDEALDNAKEALALHFEGLVADGEELPQPQAIDQHLNNPDYAGGVWAVI
EFDVTPYLGKAVRFNATLPEHLLQRIDDRVSKDQRYSSRSGFLATAALRELANS

Nucleotide

• Length: 405 bp

>AT150099 NZ_CP064387:4745617-4745865 [Escherichia coli O104:H4]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA