Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/LOTUS_5_Limkain_b1-VagC |
| Location | 3222798..3223426 | Replicon | chromosome |
| Accession | NZ_CP048830 | ||
| Organism | Leptospira interrogans serovar Copenhageni strain SK1 | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | Q8F7E4 |
| Locus tag | LICSK_RS13285 | Protein ID | WP_000281042.1 |
| Coordinates | 3223028..3223426 (+) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | Q72P17 |
| Locus tag | LICSK_RS13280 | Protein ID | WP_001192484.1 |
| Coordinates | 3222798..3223028 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LICSK_RS13250 | 3218399..3218608 | - | 210 | WP_000041838.1 | hypothetical protein | - |
| LICSK_RS13255 | 3218740..3219369 | + | 630 | WP_001081629.1 | helix-turn-helix domain-containing protein | - |
| LICSK_RS13260 | 3219576..3220499 | + | 924 | WP_000680714.1 | M28 family peptidase | - |
| LICSK_RS13265 | 3220758..3221447 | + | 690 | WP_000590460.1 | SH3 domain-containing protein | - |
| LICSK_RS13270 | 3221650..3221979 | + | 330 | WP_000611884.1 | hypothetical protein | - |
| LICSK_RS13275 | 3221976..3222506 | + | 531 | WP_000129981.1 | hypothetical protein | - |
| LICSK_RS13280 | 3222798..3223028 | + | 231 | WP_001192484.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| LICSK_RS13285 | 3223028..3223426 | + | 399 | WP_000281042.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| LICSK_RS13290 | 3223524..3223826 | + | 303 | WP_000479559.1 | hypothetical protein | - |
| LICSK_RS13295 | 3223842..3224923 | + | 1082 | Protein_2600 | IS3-like element IS1500A family transposase | - |
| LICSK_RS13300 | 3224978..3225205 | + | 228 | WP_000130601.1 | hypothetical protein | - |
| LICSK_RS13305 | 3225348..3226424 | + | 1077 | WP_001170562.1 | hypothetical protein | - |
| LICSK_RS13310 | 3226425..3226883 | + | 459 | WP_001239008.1 | GNAT family N-acetyltransferase | - |
| LICSK_RS13315 | 3227100..3227960 | - | 861 | WP_000682236.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | flank | IS/Tn | - | - | 3224075..3224923 | 848 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15139.76 Da Isoelectric Point: 9.6893
>T150096 WP_000281042.1 NZ_CP048830:3223028-3223426 [Leptospira interrogans serovar Copenhageni]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ
Download Length: 399 bp
>T150096 NZ_CP064387:c4262420-4261956 [Escherichia coli O104:H4]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8698.99 Da Isoelectric Point: 9.8940
>AT150096 WP_001192484.1 NZ_CP048830:3222798-3223028 [Leptospira interrogans serovar Copenhageni]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEGREQPKSDKREKL
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEGREQPKSDKREKL
Download Length: 231 bp
>AT150096 NZ_CP064387:c4262755-4262420 [Escherichia coli O104:H4]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0F6HAN6 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E2D7U1 |