Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1468278..1468833 Replicon chromosome
Accession NZ_CP048830
Organism Leptospira interrogans serovar Copenhageni strain SK1

Toxin (Protein)


Gene name chpK Uniprot ID Q72T28
Locus tag LICSK_RS05990 Protein ID WP_000617907.1
Coordinates 1468513..1468833 (+) Length 107 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag LICSK_RS05985 Protein ID WP_000844758.1
Coordinates 1468278..1468526 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LICSK_RS05970 1463930..1465912 + 1983 WP_000802798.1 GAF domain-containing protein -
LICSK_RS05975 1466554..1467012 - 459 WP_000453263.1 hypothetical protein -
LICSK_RS05980 1467166..1467462 + 297 WP_000477061.1 helix-turn-helix domain-containing protein -
LICSK_RS05985 1468278..1468526 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
LICSK_RS05990 1468513..1468833 + 321 WP_000617907.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LICSK_RS05995 1469833..1470825 - 993 WP_000041092.1 aldolase -
LICSK_RS06000 1470965..1471900 - 936 WP_000004530.1 ornithine carbamoyltransferase -
LICSK_RS06010 1472704..1473171 + 468 WP_000060053.1 hypothetical protein -
LICSK_RS06015 1473168..1473572 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-103)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 107 a.a.        Molecular weight: 11644.43 Da        Isoelectric Point: 9.8447

>T150095 WP_000617907.1 NZ_CP048830:1468513-1468833 [Leptospira interrogans serovar Copenhageni]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGKVEIGL

Download         Length: 321 bp

>T150095 NZ_CP064387:3937380-3937610 [Escherichia coli O104:H4]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT150095 WP_000844758.1 NZ_CP048830:1468278-1468526 [Leptospira interrogans serovar Copenhageni]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT150095 NZ_CP064387:3937607-3937966 [Escherichia coli O104:H4]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q72T28

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References