150092

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 6765..7357 Replicon plasmid pEcQE11-421-5
Accession NZ_CP048826
Organism Escherichia coli strain QEC11-421

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag QE11421_RS24890 Protein ID WP_000520549.1
Coordinates 6765..7013 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag QE11421_RS24895 Protein ID WP_000681613.1
Coordinates 7010..7357 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QE11421_RS24860 3642..4157 + 516 WP_001025395.1 J domain-containing protein -
QE11421_RS24865 4262..4804 + 543 WP_001083906.1 hypothetical protein -
QE11421_RS24870 4801..5052 + 252 WP_001230707.1 hypothetical protein -
QE11421_RS24875 5039..5266 + 228 WP_000801440.1 hypothetical protein -
QE11421_RS24880 5720..6019 + 300 WP_000650309.1 hypothetical protein -
QE11421_RS24885 6110..6460 + 351 WP_000854259.1 hypothetical protein -
QE11421_RS24890 6765..7013 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
QE11421_RS24895 7010..7357 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QE11421_RS24900 7718..8221 + 504 WP_000781813.1 hypothetical protein -
QE11421_RS24905 8225..9454 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
QE11421_RS24910 9531..9824 + 294 WP_060598542.1 LuxR family transcriptional regulator -
QE11421_RS24915 9821..10036 + 216 WP_000760375.1 EexN family lipoprotein -
QE11421_RS24920 10392..11012 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
QE11421_RS24925 11080..11352 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33309 33309


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T150092 WP_000520549.1 NZ_CP048826:6765-7013 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T150092 NZ_CP064387:c3129105-3128932 [Escherichia coli O104:H4]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT150092 WP_000681613.1 NZ_CP048826:7010-7357 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT150092 NZ_CP064387:c3129429-3129133 [Escherichia coli O104:H4]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References