Detailed information of TA system TA150091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 4801..5266
• Replicon: plasmid pEcQE11-421-5
• Accession: NZ_CP048826
• Organism: Escherichia coli strain QEC11-421

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: QE11421_RS24870
• Protein ID: WP_001230707.1
• Coordinates: 4801..5052 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: QE11421_RS24875
• Protein ID: WP_000801440.1
• Coordinates: 5039..5266 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QE11421_RS24845	282..809	-	528	WP_000577043.1	DUF2726 domain-containing protein	-
QE11421_RS24850	944..1348	-	405	WP_012421203.1	hypothetical protein	-
QE11421_RS24855	1361..2227	-	867	WP_001353743.1	replication initiation protein	-
QE11421_RS24860	3642..4157	+	516	WP_001025395.1	J domain-containing protein	-
QE11421_RS24865	4262..4804	+	543	WP_001083906.1	hypothetical protein	-
QE11421_RS24870	4801..5052	+	252	WP_001230707.1	hypothetical protein	Toxin
QE11421_RS24875	5039..5266	+	228	WP_000801440.1	hypothetical protein	Antitoxin
QE11421_RS24880	5720..6019	+	300	WP_000650309.1	hypothetical protein	-
QE11421_RS24885	6110..6460	+	351	WP_000854259.1	hypothetical protein	-
QE11421_RS24890	6765..7013	+	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
QE11421_RS24895	7010..7357	+	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
QE11421_RS24900	7718..8221	+	504	WP_000781813.1	hypothetical protein	-
QE11421_RS24905	8225..9454	+	1230	WP_001329504.1	relaxase/mobilization nuclease domain-containing protein	-
QE11421_RS24910	9531..9824	+	294	WP_060598542.1	LuxR family transcriptional regulator	-
QE11421_RS24915	9821..10036	+	216	WP_000760375.1	EexN family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33309	33309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T150091 WP_001230707.1 NZ_CP048826:4801-5052 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T150091 NZ_CP064387:3117323-3117700 [Escherichia coli O104:H4]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGAATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT150091 WP_000801440.1 NZ_CP048826:5039-5266 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT150091 NZ_CP064387:3116899-3117276 [Escherichia coli O104:H4]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACTGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9