150086

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 68387..68988 Replicon plasmid pEcQE11-421-2
Accession NZ_CP048823
Organism Escherichia coli strain QEC11-421

Toxin (Protein)


Gene name doc Uniprot ID F4TND7
Locus tag QE11421_RS23645 Protein ID WP_001216030.1
Coordinates 68608..68988 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag QE11421_RS23640 Protein ID WP_001190712.1
Coordinates 68387..68608 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QE11421_RS23630 65383..66651 - 1269 WP_029401415.1 restriction endonuclease subunit S -
QE11421_RS23635 66648..68204 - 1557 WP_029401414.1 type I restriction-modification system subunit M -
QE11421_RS23640 68387..68608 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
QE11421_RS23645 68608..68988 + 381 WP_001216030.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
QE11421_RS23650 68993..69172 + 180 WP_039022005.1 hypothetical protein -
QE11421_RS23655 69200..70069 + 870 Protein_73 DUF968 domain-containing protein -
QE11421_RS23660 70244..71095 - 852 WP_000611653.1 hypothetical protein -
QE11421_RS23665 71206..71415 - 210 WP_000874154.1 hypothetical protein -
QE11421_RS23670 71606..72303 + 698 Protein_76 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / oqxA / oqxB - 1..115329 115329


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(4-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13601.29 Da        Isoelectric Point: 5.1408

>T150086 WP_001216030.1 NZ_CP048823:68608-68988 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEDITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T150086 NZ_CP064387:c1484911-1484537 [Escherichia coli O104:H4]
ATGAAATTATTACCCGACACACATGTACGGGAGGCATCATGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGTCTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT150086 WP_001190712.1 NZ_CP048823:68387-68608 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT150086 NZ_CP064387:c1485355-1485002 [Escherichia coli O104:H4]
GTGTCAGACACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGCGC
CCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATG
CGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTGAATCCCCGC
CATCAGCATACCGTCACGCTATATGCTAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTGTGGTTACGTTTATCT
GGCTGTTTATCCGACCCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1M1W8Q3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References