Detailed information of TA system TA150080

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 22035..22582
• Replicon: plasmid pEcQE11-421-1
• Accession: NZ_CP048822
• Organism: Escherichia coli strain QEC11-421

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: QE11421_RS22620
• Protein ID: WP_000969990.1
• Coordinates: 22035..22316 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E1QMW3
• Locus tag: QE11421_RS22625
• Protein ID: WP_000079941.1
• Coordinates: 22313..22582 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QE11421_RS22600	18300..19334	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
QE11421_RS22605	19893..20240	-	348	WP_000142437.1	hypothetical protein	-
QE11421_RS22610	20542..21024	-	483	WP_001311056.1	hypothetical protein	-
QE11421_RS22615	21141..21989	-	849	WP_001058005.1	3'-5' exonuclease	-
QE11421_RS22620	22035..22316	-	282	WP_000969990.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QE11421_RS22625	22313..22582	-	270	WP_000079941.1	hypothetical protein	Antitoxin
QE11421_RS22630	23104..24340	+	1237	Protein_19	IS3 family transposase	-
QE11421_RS22635	24537..25811	+	1275	WP_000489607.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat	1..137410	137410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10930.61 Da
• Isoelectric Point: 8.4615

>T150080 WP_000969990.1 NZ_CP048822:c22316-22035 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASNLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTVQKWP

Nucleotide

• Length: 282 bp

>T150080 NZ_CP048822:c22316-22035 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAACCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGTACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10252.58 Da
• Isoelectric Point: 7.2057

>AT150080 WP_000079941.1 NZ_CP048822:c22582-22313 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT150080 NZ_CP048822:c22582-22313 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATTGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTACTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A629TXC5