Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4157082..4157700 Replicon chromosome
Accession NZ_CP048821
Organism Escherichia coli strain QEC11-421

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QE11421_RS20350 Protein ID WP_001291435.1
Coordinates 4157082..4157300 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QE11421_RS20355 Protein ID WP_000344800.1
Coordinates 4157326..4157700 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QE11421_RS20315 4152371..4152943 + 573 WP_063117855.1 YbaY family lipoprotein -
QE11421_RS20320 4152974..4153285 - 312 WP_000409911.1 MGMT family protein -
QE11421_RS20330 4153664..4154017 + 354 WP_000878140.1 DUF1428 domain-containing protein -
QE11421_RS20335 4154059..4155609 - 1551 WP_199265998.1 EAL domain-containing protein -
QE11421_RS20340 4155773..4156243 - 471 WP_000136192.1 YlaC family protein -
QE11421_RS20345 4156359..4156910 - 552 WP_000102564.1 maltose O-acetyltransferase -
QE11421_RS20350 4157082..4157300 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
QE11421_RS20355 4157326..4157700 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QE11421_RS20360 4158246..4161395 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
QE11421_RS20365 4161418..4162611 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T150079 WP_001291435.1 NZ_CP048821:c4157300-4157082 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T150079 NZ_CP064387:256191-256445 [Escherichia coli O104:H4]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT150079 WP_000344800.1 NZ_CP048821:c4157700-4157326 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT150079 NZ_CP064387:255943-256194 [Escherichia coli O104:H4]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References