Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3609520..3610096 Replicon chromosome
Accession NZ_CP048821
Organism Escherichia coli strain QEC11-421

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QE11421_RS17535 Protein ID WP_001297643.1
Coordinates 3609520..3609807 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QE11421_RS17540 Protein ID WP_000063156.1
Coordinates 3609794..3610096 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QE11421_RS17515 3605773..3607053 - 1281 WP_001037380.1 DUF445 domain-containing protein -
QE11421_RS17520 3607094..3608326 - 1233 WP_063117540.1 multidrug efflux MFS transporter MdtM -
QE11421_RS17525 3608472..3608567 - 96 Protein_3413 dienelactone hydrolase family protein -
QE11421_RS17530 3608624..3609321 + 698 Protein_3414 IS1 family transposase -
QE11421_RS17535 3609520..3609807 + 288 WP_001297643.1 BrnT family toxin Toxin
QE11421_RS17540 3609794..3610096 + 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QE11421_RS17545 3610130..3611086 - 957 WP_001297640.1 GTPase -
QE11421_RS17550 3611097..3611300 - 204 WP_000467859.1 YbdD/YjiX family protein -
QE11421_RS17555 3611350..3613500 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T150077 WP_001297643.1 NZ_CP048821:3609520-3609807 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T150077 NZ_CP064386:43176-43574 [Salmonella enterica subsp. enterica]
ATGCTGAAGTTTATGCTGGATACTAACATCTGCATTTTCACGATAAAAAACAAACCTGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCGGTCACCCTGATGGAGCTGATTTACGGCGCCGAAAAAAGCCAGATGCCCG
AAAGAAATCTCGCTGTGATAGAGGGGTTTGTTTCCCGACTTGATGTTCTGGATTATGACACGCCGGCCGCCACCCATACG
GGGCAGATCAGGGCAGAGCTTGCCCGTCAGGGACGCCCTGTCGGGCCATTCGATCAGATGATTGCCGGTCACGCACGCAG
CCGGGGACTGATTGTTGTCACGAACAACACCCGGGAGTTTGAACGTGTGGGTGGACTGAGAACTGAGGACTGGAGCTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT150077 WP_000063156.1 NZ_CP048821:3609794-3610096 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT150077 NZ_CP064386:42949-43176 [Salmonella enterica subsp. enterica]
ATGGAAACCAGCGTATTTCTCAGTAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCAGTGGCTTTGCCGGAAAATGTAAA
ACGTGTGGAAGTTATCGCTGTCGGGCGAACCCGAATTATCACGCCGGCCGGAGAATCGTGGGATGAATGGTTCGACGGGA
ACAACGTCAGCGCCGATTTTATGGATAACCGGGAACAACCCTCTGTGCAGGAACGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References