Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3426821..3427318 Replicon chromosome
Accession NZ_CP048821
Organism Escherichia coli strain QEC11-421

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QE11421_RS16630 Protein ID WP_001173343.1
Coordinates 3427145..3427318 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QE11421_RS16625 Protein ID WP_000398619.1
Coordinates 3426821..3427117 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QE11421_RS16605 3423552..3424271 - 720 WP_000611288.1 two-component system response regulator DcuR -
QE11421_RS16610 3424268..3425899 - 1632 WP_001216477.1 sensor histidine kinase -
QE11421_RS16615 3426080..3426310 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
QE11421_RS16620 3426322..3426594 + 273 WP_000405651.1 N-acetyltransferase -
QE11421_RS16625 3426821..3427117 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QE11421_RS16630 3427145..3427318 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QE11421_RS16635 3427437..3428954 - 1518 WP_001295074.1 lysine--tRNA ligase -
QE11421_RS16640 3429191..3430648 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T150076 WP_001173343.1 NZ_CP048821:3427145-3427318 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T150076 NZ_CP064386:c15894-15589 [Salmonella enterica subsp. enterica]
ATGCAGTTTAAGGTTTACACCTGTAAAAGGGAGAGTCGCTACCGGCTGTTTGTGGACGTGCAGAGCGACATCATTGACAC
CCCCGGACGGCGGATGGCTGTCCCGCTGGTCAGCGCCCGTCTGCTGTCGGAAAAGGTTCCCCGCGATCTTTACCCGGTGA
TGCATATCGGGGATGAGCCTTACCGCCTGCTGACCACGGATATGACCAGTGTGCCGGCCACCGTCATCGGGGAAGAGGTG
GCCGATCTCAGCCTCCGGGAGAACGATATCAAAAACGCCATTAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT150076 WP_000398619.1 NZ_CP048821:3426821-3427117 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT150076 NZ_CP064386:c16114-15896 [Salmonella enterica subsp. enterica]
ATGAAGCAGCGAATTACAGTGACAGTGGACAGCGACAGCTATCAGCTGCTCAAGGCATACGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGAGCCGAACGCTGGCAGGAAGAAAATCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATAGAGGCTAACGGATCGTTTGCTGACGACAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References