Detailed information of TA system TA150071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1982067..1982650
• Replicon: chromosome
• Accession: NZ_CP048821
• Organism: Escherichia coli strain QEC11-421

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: QE11421_RS09765
• Protein ID: WP_049253269.1
• Coordinates: 1982067..1982402 (-)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A1V2T5M9
• Locus tag: QE11421_RS09770
• Protein ID: WP_000581941.1
• Coordinates: 1982402..1982650 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QE11421_RS09750	1977954..1979252	-	1299	WP_000036723.1	phosphopyruvate hydratase	-
QE11421_RS09755	1979340..1980977	-	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
QE11421_RS09760	1981205..1981996	-	792	WP_001071648.1	nucleoside triphosphate pyrophosphohydrolase	-
QE11421_RS09765	1982067..1982402	-	336	WP_049253269.1	endoribonuclease MazF	Toxin
QE11421_RS09770	1982402..1982650	-	249	WP_000581941.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
QE11421_RS09775	1982728..1984962	-	2235	WP_000226815.1	GTP pyrophosphokinase	-
QE11421_RS09780	1985010..1986311	-	1302	WP_023281448.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12197.18 Da
• Isoelectric Point: 8.7218

>T150071 WP_049253269.1 NZ_CP048821:c1982402-1982067 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQARHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T150071 NZ_CP064385:3308486-3308977 [Salmonella enterica subsp. enterica]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGGAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.76 Da
• Isoelectric Point: 4.4665

>AT150071 WP_000581941.1 NZ_CP048821:c1982650-1982402 [Escherichia coli]
MIHSSVKRWGNSPAVRIPTTLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT150071 NZ_CP064385:3308196-3308489 [Salmonella enterica subsp. enterica]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A1V2T5M9