Detailed information of TA system TA150061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 622113..623701
• Replicon: chromosome
• Accession: NZ_CP048821
• Organism: Escherichia coli strain QEC11-421

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: QE11421_RS03025
• Protein ID: WP_063117624.1
• Coordinates: 622113..623435 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: QE11421_RS03030
• Protein ID: WP_001301023.1
• Coordinates: 623435..623701 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QE11421_RS02995	617716..618219	-	504	WP_000825452.1	type 1 fimbrial protein	-
QE11421_RS03000	618232..618762	-	531	WP_000876763.1	type 1 fimbrial protein	-
QE11421_RS03005	618776..619654	-	879	Protein_591	fimbrial biogenesis outer membrane usher protein	-
QE11421_RS03010	619739..620967	+	1229	WP_089541817.1	IS3 family transposase	-
QE11421_RS03015	620979..621338	-	360	Protein_593	fimbria/pilus outer membrane usher protein	-
QE11421_RS03020	621593..621801	-	209	Protein_594	helix-turn-helix transcriptional regulator	-
QE11421_RS03025	622113..623435	-	1323	WP_063117624.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
QE11421_RS03030	623435..623701	-	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
QE11421_RS03035	623924..625324	-	1401	WP_063117625.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49291.81 Da
• Isoelectric Point: 8.2482

>T150061 WP_063117624.1 NZ_CP048821:c623435-622113 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGSVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T150061 NZ_CP064385:c372421-372137 [Salmonella enterica subsp. enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGGCTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT150061 WP_001301023.1 NZ_CP048821:c623701-623435 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT150061 NZ_CP064385:c372659-372411 [Salmonella enterica subsp. enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGCAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA