Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 543447..544085 Replicon chromosome
Accession NZ_CP048821
Organism Escherichia coli strain QEC11-421

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag QE11421_RS02685 Protein ID WP_000813794.1
Coordinates 543447..543623 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag QE11421_RS02690 Protein ID WP_001270286.1
Coordinates 543669..544085 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QE11421_RS02665 539066..540280 - 1215 WP_071597387.1 BenE family transporter YdcO -
QE11421_RS02670 540333..540869 + 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
QE11421_RS02675 540942..542903 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
QE11421_RS02680 542995..543225 - 231 WP_000494244.1 YncJ family protein -
QE11421_RS02685 543447..543623 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
QE11421_RS02690 543669..544085 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
QE11421_RS02695 544164..545570 + 1407 WP_199266048.1 PLP-dependent aminotransferase family protein -
QE11421_RS02700 545815..546960 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
QE11421_RS02705 546978..547991 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
QE11421_RS02710 547992..548933 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 537761..539026 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T150060 WP_000813794.1 NZ_CP048821:543447-543623 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T150060 NZ_CP064384:79658-79933 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT150060 WP_001270286.1 NZ_CP048821:543669-544085 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT150060 NZ_CP064384:79374-79658 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References