Detailed information of TA system TA150050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 1607157..1607791
• Replicon: chromosome
• Accession: NZ_CP048815
• Organism: Caulobacter rhizosphaerae strain KCTC 52515

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G3M57_RS07650
• Protein ID: WP_163229776.1
• Coordinates: 1607157..1607561 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: G3M57_RS07655
• Protein ID: WP_056757115.1
• Coordinates: 1607558..1607791 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M57_RS07620	1602620..1603330	-	711	WP_056757134.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
G3M57_RS07625	1603327..1603950	-	624	WP_056757130.1	YdcF family protein	-
G3M57_RS07630	1603947..1604837	-	891	WP_056757128.1	hypothetical protein	-
G3M57_RS07635	1604834..1605535	-	702	WP_163233681.1	cell division ATP-binding protein FtsE	-
G3M57_RS07640	1605682..1606617	+	936	WP_163229774.1	zinc-ribbon domain-containing protein	-
G3M57_RS07645	1606633..1607160	+	528	WP_056757121.1	hypoxanthine phosphoribosyltransferase	-
G3M57_RS07650	1607157..1607561	-	405	WP_163229776.1	type II toxin-antitoxin system VapC family toxin	Toxin
G3M57_RS07655	1607558..1607791	-	234	WP_056757115.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
G3M57_RS07660	1607853..1609118	-	1266	WP_056757112.1	diaminopimelate decarboxylase	-
G3M57_RS07665	1609268..1609591	-	324	WP_056757108.1	hypothetical protein	-
G3M57_RS07670	1609570..1610988	-	1419	WP_163229778.1	argininosuccinate lyase	-
G3M57_RS07675	1610989..1611618	+	630	WP_163229780.1	TlpA family protein disulfide reductase	-
G3M57_RS07680	1611808..1612011	+	204	WP_007668086.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-125)

Antitoxin

• SpoVT-AbrB domain (10-57)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14766.84 Da
• Isoelectric Point: 6.3322

>T150050 WP_163229776.1 NZ_CP048815:c1607561-1607157 [Caulobacter rhizosphaerae]
MSQGWLLDTNILSDLVRNPSGRVAEQIRRCGESHVRTSIVVAAELQFGAAKKGSERLTHQLETILGAIEVLPFTAPADHL
YGELRTKLEKAGKPIGGNDMLIAAHALASDHILVTDNEREFSRIEGLTIENWLR

Nucleotide

• Length: 405 bp

>T150050 NZ_CP064383:3955165-3955650 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8612.81 Da
• Isoelectric Point: 4.3525

>AT150050 WP_056757115.1 NZ_CP048815:c1607791-1607558 [Caulobacter rhizosphaerae]
MGYEKHVKLFRNGRNQAVRIPRELELPGDEAILRKDGDRLILEPAPPRSLLAVLASLQPIDDDFGAIDDPEPEPVDL

Nucleotide

• Length: 234 bp

>AT150050 NZ_CP064383:3954896-3955174 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA