Detailed information of TA system TA150048

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 4524971..4525506
• Replicon: chromosome
• Accession: NZ_CP048814
• Organism: Rhodococcus triatomae strain DSM 44892

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: G4H71_RS21490
• Protein ID: WP_072740057.1
• Coordinates: 4525177..4525506 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: G4H71_RS21485
• Protein ID: WP_072740058.1
• Coordinates: 4524971..4525180 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4H71_RS21455	4520353..4520931	-	579	WP_083343121.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
G4H71_RS21460	4521084..4521539	+	456	WP_139183266.1	DUF2628 domain-containing protein	-
G4H71_RS21465	4521647..4522402	+	756	WP_083343120.1	DUF4111 domain-containing protein	-
G4H71_RS21470	4522438..4523148	+	711	WP_072740060.1	hypothetical protein	-
G4H71_RS21475	4523197..4523631	-	435	WP_072740059.1	MarR family transcriptional regulator	-
G4H71_RS21480	4523709..4524725	+	1017	WP_083343102.1	NADP-dependent oxidoreductase	-
G4H71_RS21485	4524971..4525180	+	210	WP_072740058.1	DUF3018 family protein	Antitoxin
G4H71_RS21490	4525177..4525506	+	330	WP_072740057.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
G4H71_RS21495	4526019..4526867	+	849	WP_072740056.1	alpha/beta hydrolase	-
G4H71_RS21500	4526901..4528334	-	1434	WP_072740055.1	condensation protein	-
G4H71_RS21505	4528334..4528780	-	447	WP_072740054.1	hypothetical protein	-
G4H71_RS21510	4528777..4529433	-	657	WP_072740053.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-104)

Antitoxin

• Antitoxin MazE-like (4-67)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11924.88 Da
• Isoelectric Point: 5.1213

>T150048 WP_072740057.1 NZ_CP048814:4525177-4525506 [Rhodococcus triatomae]
VIRGEVWTVTGDVYASKPRPAVIIQDDLFDATLSVVVAPMTSNLIEAPLMRIRIPAGGELSGLDRDSDVMVDKMTAVRRS
NVLTRVGRLTSEQLVEVERSLMAFLGLAR

Nucleotide

• Length: 330 bp

>T150048 NZ_CP064383:c3661017-3660703 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7884.62 Da
• Isoelectric Point: 4.7640

>AT150048 WP_072740058.1 NZ_CP048814:4524971-4525180 [Rhodococcus triatomae]
VAVRDRVGDYRRRMRERGLRPLQVWVPDVRTATFAAEAHRQSALVAATDSHGDEQDFVDSIATSWDDEE

Nucleotide

• Length: 210 bp

>AT150048 NZ_CP064383:c3660706-3660290 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA