Detailed information of TA system TA150035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 501836..502472
• Replicon: chromosome
• Accession: NZ_CP048811
• Organism: Bacillus altitudinis strain SCU11

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: K2MHT4
• Locus tag: G4D59_RS02420
• Protein ID: WP_003214169.1
• Coordinates: 502122..502472 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W8QJ31
• Locus tag: G4D59_RS02415
• Protein ID: WP_003214273.1
• Coordinates: 501836..502117 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4D59_RS02395 (G4D59_02390)	497990..498595	-	606	WP_007496491.1	rhomboid family intramembrane serine protease	-
G4D59_RS02400 (G4D59_02395)	498690..499055	+	366	WP_017366663.1	holo-ACP synthase	-
G4D59_RS02405 (G4D59_02400)	499216..500232	+	1017	WP_007496489.1	outer membrane lipoprotein-sorting protein	-
G4D59_RS02410 (G4D59_02405)	500361..501542	+	1182	WP_017366664.1	alanine racemase	-
G4D59_RS02415 (G4D59_02410)	501836..502117	+	282	WP_003214273.1	hypothetical protein	Antitoxin
G4D59_RS02420 (G4D59_02415)	502122..502472	+	351	WP_003214169.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
G4D59_RS02425 (G4D59_02420)	502589..503419	+	831	WP_008346908.1	RsbT co-antagonist protein RsbRA	-
G4D59_RS02430 (G4D59_02425)	503424..503792	+	369	WP_003214235.1	RsbT antagonist protein RsbS	-
G4D59_RS02435 (G4D59_02430)	503795..504196	+	402	WP_003214085.1	anti-sigma regulatory factor	-
G4D59_RS02440 (G4D59_02435)	504207..505214	+	1008	WP_008346904.1	PP2C family protein-serine/threonine phosphatase	-
G4D59_RS02445 (G4D59_02440)	505274..505603	+	330	WP_008346902.1	anti-sigma factor antagonist	-
G4D59_RS02450 (G4D59_02445)	505600..506088	+	489	WP_007496471.1	anti-sigma B factor RsbW	-
G4D59_RS02455 (G4D59_02450)	506054..506842	+	789	WP_012009000.1	RNA polymerase sigma factor SigB	-
G4D59_RS02460 (G4D59_02455)	506842..507441	+	600	WP_008346897.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12976.99 Da
• Isoelectric Point: 5.1663

>T150035 WP_003214169.1 NZ_CP048811:502122-502472 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Nucleotide

• Length: 351 bp

>T150035 NZ_CP064377:c5933402-5933124 [Achromobacter xylosoxidans]
ATGCTCGACGTATCCTGGCTTCAAACCGCCGCAGACGATCTGGCGGGGATCATCTCCTACATCGCCGAGCGTAGTCCGCA
GGCGGCACGCGACCTCCGGCAACGCATCGACAATGCCGTGCTGTCCCTGGCGCGGCACCCTTGCCGATATCGAGCCGGCC
GCGTCTCTGGCACGCGCGAATGCGTCGTACACCCTAATTACGTGGTGGTCTACCGGGTGACCGCGTTGGCGATCGAGGTC
GTCAACATCGTGCACGCCCGCCGGGAATATCCCCCCTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10864.25 Da
• Isoelectric Point: 5.2726

>AT150035 WP_003214273.1 NZ_CP048811:501836-502117 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT150035 NZ_CP064377:c5933678-5933472 [Achromobacter xylosoxidans]
ATGGGCCCAATCCCTTCCTCCATCCTCTCGGAATTCGAGACCGAAGAACAGGCGGTCAGCTACGACCGCTGGTTCCGCGC
GAAGGTGCAAGCCAGCCTGGACGATCCGCGTCCCGCCGTTTCGCATCAAAGGGTGATGGCCCGCATCGATGCCATTATCG
AAACGGCGTGCCGTCGTCAGCTGGATCCGCAAGACGGAGAAAACTGA

Structures

Toxin

• AlphaFold DB: K2MHT4

Antitoxin

• AlphaFold DB: A0A081L854