Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 93975..94618 Replicon plasmid pEGKP4.1
Accession NZ_CP048801
Organism Klebsiella pneumoniae strain EGKP4

Toxin (Protein)


Gene name vagD Uniprot ID A0A7D6UII7
Locus tag G4I41_RS26795 Protein ID WP_032425563.1
Coordinates 93975..94391 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag G4I41_RS26800 Protein ID WP_001261276.1
Coordinates 94388..94618 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4I41_RS26780 (G4I41_27220) 89019..91127 - 2109 WP_265430391.1 peptidase domain-containing ABC transporter -
G4I41_RS26785 (G4I41_27225) 91117..92394 - 1278 WP_265430392.1 HlyD family secretion protein -
G4I41_RS26790 (G4I41_27230) 93056..93934 + 879 WP_009309916.1 restriction endonuclease -
G4I41_RS26795 (G4I41_27235) 93975..94391 - 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin Toxin
G4I41_RS26800 (G4I41_27240) 94388..94618 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
G4I41_RS26805 (G4I41_27245) 94671..95045 + 375 WP_171483593.1 hypothetical protein -
G4I41_RS26810 (G4I41_27255) 95206..96150 + 945 WP_011977810.1 hypothetical protein -
G4I41_RS26815 (G4I41_27260) 96187..96579 + 393 WP_011977811.1 hypothetical protein -
G4I41_RS26820 (G4I41_27265) 96637..97158 + 522 WP_013214008.1 hypothetical protein -
G4I41_RS26825 (G4I41_27270) 97204..97407 + 204 WP_011977813.1 hypothetical protein -
G4I41_RS26830 (G4I41_27275) 97437..98441 + 1005 WP_011977814.1 hypothetical protein -
G4I41_RS26835 (G4I41_27285) 98625..99404 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / blaSHV-12 / blaOXA-10 / qacE / sul1 - 1..192902 192902


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15080.56 Da        Isoelectric Point: 8.5403

>T150027 WP_032425563.1 NZ_CP048801:c94391-93975 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T150027 NZ_CP064376:c3380150-3379977 [Shigella sonnei]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT150027 WP_001261276.1 NZ_CP048801:c94618-94388 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT150027 NZ_CP064376:c3380474-3380178 [Shigella sonnei]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6UII7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References