Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 54264..55000 Replicon plasmid pEGKP4.1
Accession NZ_CP048801
Organism Klebsiella pneumoniae strain EGKP4

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag G4I41_RS26540 Protein ID WP_003026803.1
Coordinates 54264..54746 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag G4I41_RS26545 Protein ID WP_003026799.1
Coordinates 54734..55000 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4I41_RS26515 (G4I41_26960) 49544..50743 + 1200 WP_069484144.1 tetracycline efflux MFS transporter Tet(A) -
G4I41_RS26520 (G4I41_26965) 50775..51659 - 885 WP_000058717.1 EamA family transporter -
G4I41_RS26525 (G4I41_26970) 51797..52189 - 393 WP_001351729.1 isochorismatase family cysteine hydrolase -
G4I41_RS26530 (G4I41_26975) 52193..53944 + 1752 Protein_55 Tn3-like element TnAs1 family transposase -
G4I41_RS26535 (G4I41_26980) 53978..54148 - 171 Protein_56 ISNCY family transposase -
G4I41_RS26540 (G4I41_26985) 54264..54746 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
G4I41_RS26545 (G4I41_26990) 54734..55000 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
G4I41_RS26550 (G4I41_26995) 55297..57589 - 2293 Protein_59 Tn3 family transposase -
G4I41_RS26555 (G4I41_27000) 57748..58326 + 579 WP_040229494.1 recombinase family protein -
G4I41_RS26560 (G4I41_27005) 58502..58930 + 429 Protein_61 chromate transporter -
G4I41_RS26565 (G4I41_27010) 58998..59702 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
G4I41_RS26570 59756..59905 - 150 Protein_63 class II aldolase/adducin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / blaSHV-12 / blaOXA-10 / qacE / sul1 - 1..192902 192902
- inside IScluster/Tn tet(A) / blaSHV-12 / blaOXA-10 / qacE / sul1 - 45397..69776 24379


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T150025 WP_003026803.1 NZ_CP048801:c54746-54264 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T150025 NZ_CP064376:2678734-2679276 [Shigella sonnei]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT150025 WP_003026799.1 NZ_CP048801:c55000-54734 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT150025 NZ_CP064376:2678439-2678750 [Shigella sonnei]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References