Detailed information of TA system TA150023

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4951275..4952956
• Replicon: chromosome
• Accession: NZ_CP048800
• Organism: Klebsiella pneumoniae strain EGKP4

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: G4I41_RS24440
• Protein ID: WP_004146663.1
• Coordinates: 4951275..4952192 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: G4I41_RS24445
• Protein ID: WP_004177809.1
• Coordinates: 4952198..4952956 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4I41_RS24420 (4946581)	4946581..4948101	+	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-
G4I41_RS24425 (4948098)	4948098..4949096	+	999	WP_012737175.1	ribose ABC transporter permease	-
G4I41_RS24430 (4949127)	4949127..4950068	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
G4I41_RS24435 (4950143)	4950143..4951150	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
G4I41_RS24440 (4951275)	4951275..4952192	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
G4I41_RS24445 (4952198)	4952198..4952956	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
G4I41_RS24450 (4953042)	4953042..4953770	-	729	WP_002885152.1	response regulator transcription factor	-
G4I41_RS24455 (4954043)	4954043..4956190	+	2148	WP_238952516.1	formate dehydrogenase subunit alpha	-
G4I41_RS24460 (4956420)	4956420..4957106	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T150023 WP_004146663.1 NZ_CP048800:c4952192-4951275 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T150023 NZ_CP064376:c1712911-1712808 [Shigella sonnei]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT150023 WP_004177809.1 NZ_CP048800:c4952956-4952198 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT150023 NZ_CP064376:1712807-1713041 [Shigella sonnei]
TAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCA
TTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAAT
AAAAAGCGCGGTGGTCATCAGCTTAAATGATAAAAACCGCCCGTTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05