Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4686624..4687434 Replicon chromosome
Accession NZ_CP048800
Organism Klebsiella pneumoniae strain EGKP4

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag G4I41_RS23155 Protein ID WP_014908042.1
Coordinates 4686624..4687157 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID -
Locus tag G4I41_RS23160 Protein ID WP_023343051.1
Coordinates 4687168..4687434 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4I41_RS23150 (4685455) 4685455..4686576 + 1122 WP_012737335.1 cupin domain-containing protein -
G4I41_RS23155 (4686624) 4686624..4687157 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
G4I41_RS23160 (4687168) 4687168..4687434 - 267 WP_023343051.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
G4I41_RS23165 (4687537) 4687537..4688970 - 1434 WP_023343050.1 Cu(+)/Ag(+) sensor histidine kinase -
G4I41_RS23170 (4688960) 4688960..4689643 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
G4I41_RS23175 (4689816) 4689816..4691201 + 1386 WP_024623207.1 efflux transporter outer membrane subunit -
G4I41_RS23180 (4691219) 4691219..4691563 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T150021 WP_014908042.1 NZ_CP048800:c4687157-4686624 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T150021 NZ_CP064376:c973450-973343 [Shigella sonnei]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10021.46 Da        Isoelectric Point: 10.5807

>AT150021 WP_023343051.1 NZ_CP048800:c4687434-4687168 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT150021 NZ_CP064376:973495-973561 [Shigella sonnei]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure

References