Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4091816..4092435 Replicon chromosome
Accession NZ_CP048800
Organism Klebsiella pneumoniae strain EGKP4

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag G4I41_RS20300 Protein ID WP_002892050.1
Coordinates 4092217..4092435 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag G4I41_RS20295 Protein ID WP_002892066.1
Coordinates 4091816..4092190 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4I41_RS20285 (4086968) 4086968..4088161 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
G4I41_RS20290 (4088184) 4088184..4091330 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
G4I41_RS20295 (4091816) 4091816..4092190 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
G4I41_RS20300 (4092217) 4092217..4092435 + 219 WP_002892050.1 HHA domain-containing protein Toxin
G4I41_RS20305 (4092594) 4092594..4093160 + 567 WP_002892030.1 maltose O-acetyltransferase -
G4I41_RS20310 (4093132) 4093132..4093272 - 141 WP_004147370.1 hypothetical protein -
G4I41_RS20315 (4093293) 4093293..4093763 + 471 WP_002892026.1 YlaC family protein -
G4I41_RS20320 (4093738) 4093738..4095189 - 1452 WP_023302231.1 PLP-dependent aminotransferase family protein -
G4I41_RS20325 (4095290) 4095290..4095988 + 699 WP_002892021.1 GNAT family protein -
G4I41_RS20330 (4095985) 4095985..4096125 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
G4I41_RS20335 (4096125) 4096125..4096388 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T150020 WP_002892050.1 NZ_CP048800:4092217-4092435 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T150020 NZ_CP064376:c972914-972807 [Shigella sonnei]
ATGACGCTCGCGAAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT150020 WP_002892066.1 NZ_CP048800:4091816-4092190 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT150020 NZ_CP064376:972967-973028 [Shigella sonnei]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References