Detailed information of TA system TA150019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2691696..2693284
• Replicon: chromosome
• Accession: NZ_CP048800
• Organism: Klebsiella pneumoniae strain EGKP4

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486S9S0
• Locus tag: G4I41_RS13270
• Protein ID: WP_023301940.1
• Coordinates: 2691696..2693018 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: G4I41_RS13275
• Protein ID: WP_024622638.1
• Coordinates: 2693018..2693284 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4I41_RS13255 (2686752)	2686752..2689253	+	2502	WP_023301938.1	fimbria/pilus outer membrane usher protein	-
G4I41_RS13260 (2689241)	2689241..2690245	+	1005	WP_023301939.1	fimbrial protein	-
G4I41_RS13265 (2690387)	2690387..2691679	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
G4I41_RS13270 (2691696)	2691696..2693018	-	1323	WP_023301940.1	type II toxin-antitoxin system HipA family toxin	Toxin
G4I41_RS13275 (2693018)	2693018..2693284	-	267	WP_024622638.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
G4I41_RS13280 (2693549)	2693549..2693875	-	327	WP_004184023.1	hydroxyisourate hydrolase	-
G4I41_RS13285 (2693872)	2693872..2694372	-	501	WP_004184022.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
G4I41_RS13290 (2694350)	2694350..2695729	-	1380	WP_023301941.1	nucleobase:cation symporter-2 family protein	-
G4I41_RS13295 (2696075)	2696075..2697229	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
G4I41_RS13300 (2697210)	2697210..2698133	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49185.49 Da
• Isoelectric Point: 7.5358

>T150019 WP_023301940.1 NZ_CP048800:c2693018-2691696 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T150019 NZ_CP064376:c972379-972272 [Shigella sonnei]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9924.30 Da
• Isoelectric Point: 8.5912

>AT150019 WP_024622638.1 NZ_CP048800:c2693284-2693018 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYMKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT150019 NZ_CP064376:972427-972493 [Shigella sonnei]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTC

Structures

Toxin

• AlphaFold DB: A0A486S9S0