Detailed information of TA system TA150007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 4228601..4229233
• Replicon: chromosome
• Accession: NZ_CP048799
• Organism: Brevibacillus sp. 7WMA2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0F7EEZ8
• Locus tag: GOP56_RS18105
• Protein ID: WP_003333534.1
• Coordinates: 4228883..4229233 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GOP56_RS18100
• Protein ID: WP_173645602.1
• Coordinates: 4228601..4228879 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOP56_RS18075	4224335..4224667	+	333	WP_003333541.1	metalloregulator ArsR/SmtB family transcription factor	-
GOP56_RS18080	4224878..4225204	+	327	WP_121473327.1	gamma-type small acid-soluble spore protein	-
GOP56_RS18085	4225526..4226557	+	1032	WP_003333538.1	DUF4367 domain-containing protein	-
GOP56_RS18090	4226783..4227226	+	444	WP_163246211.1	hypothetical protein	-
GOP56_RS18095	4227230..4228435	+	1206	WP_163246212.1	alanine racemase	-
GOP56_RS18100	4228601..4228879	+	279	WP_173645602.1	ribbon-helix-helix protein, CopG family	Antitoxin
GOP56_RS18105	4228883..4229233	+	351	WP_003333534.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GOP56_RS18110	4229463..4231622	+	2160	WP_163246213.1	RNA-binding transcriptional accessory protein	-
GOP56_RS18115	4231694..4231819	-	126	WP_003333532.1	cortex morphogenetic protein CmpA	-
GOP56_RS18120	4231931..4232266	-	336	WP_022586369.1	hypothetical protein	-
GOP56_RS18125	4232385..4232849	+	465	WP_003333530.1	SprT family protein	-
GOP56_RS18130	4232915..4233385	+	471	WP_064019190.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE	-
GOP56_RS18135	4233366..4234079	+	714	WP_163246214.1	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (8-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12932.83 Da
• Isoelectric Point: 4.8553

>T150007 WP_003333534.1 NZ_CP048799:4228883-4229233 [Brevibacillus sp. 7WMA2]
VNVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKTYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMERVNESLQISLGLIDF

Nucleotide

• Length: 351 bp

>T150007 NZ_CP064374:694802-694951 [Enterococcus faecalis]
ATGCGTATGCACGTATTTCCGAAATTTAGAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACAATTCTTGG
GTTTGGTATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10729.43 Da
• Isoelectric Point: 9.6543

>AT150007 WP_173645602.1 NZ_CP048799:4228601-4228879 [Brevibacillus sp. 7WMA2]
MSKSNTKRIMISLPQHLLQEVDGVVQKERSNRSELIRQAMSLYLKERKKRFIRETMQRGYLEMAKINLNMASEAFEAEEE
ADLTLDRLVSGV

Nucleotide

• Length: 279 bp

>AT150007 NZ_CP064374:c695068-694884 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGCGCA
ATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A0F7EEZ8