Detailed information of TA system TA150002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 848132..848782
• Replicon: chromosome
• Accession: NZ_CP048796
• Organism: Providencia vermicola strain P8538

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: K8W3H1
• Locus tag: G3341_RS03630
• Protein ID: WP_004927064.1
• Coordinates: 848132..848335 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: G3341_RS03635
• Protein ID: WP_154635730.1
• Coordinates: 848414..848782 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3341_RS03605	844012..844350	+	339	WP_154622397.1	P-II family nitrogen regulator	-
G3341_RS03610	844361..845644	+	1284	WP_154622396.1	ammonium transporter AmtB	-
G3341_RS03615	845872..846738	-	867	WP_154622395.1	acyl-CoA thioesterase II	-
G3341_RS03620	847063..847521	+	459	WP_154622394.1	YbaY family lipoprotein	-
G3341_RS03630	848132..848335	-	204	WP_004927064.1	hemolysin expression modulator Hha	Toxin
G3341_RS03635	848414..848782	-	369	WP_154635730.1	Hha toxicity modulator TomB	Antitoxin
G3341_RS03640	849350..850687	-	1338	WP_154624041.1	murein transglycosylase D	-
G3341_RS03645	850766..851521	-	756	WP_163860719.1	hydroxyacylglutathione hydrolase	-
G3341_RS03650	851561..852286	+	726	WP_154624043.1	methyltransferase domain-containing protein	-
G3341_RS03655	852353..852823	-	471	WP_154624044.1	ribonuclease HI	-
G3341_RS03660	852878..853639	+	762	WP_154624045.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8075.37 Da
• Isoelectric Point: 6.9770

>T150002 WP_004927064.1 NZ_CP048796:c848335-848132 [Providencia vermicola]
MTKTDYLMRLRKCTTIDTLERVIEKNKYELSEDELELFYSAADHRLAELTMNKLYDKIPASVWKFVR

Nucleotide

• Length: 204 bp

>T150002 NZ_CP064374:393112-393456 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTTGAAGAATATAATGTGGAGTTGGTCTTTACTACATTACACAAAAAAGCTTGCTTCGA
ATCAGAACATGGTGTGATTTTTGTTAATCAAAATTTATCTACTGAAGAACAAGAAGAAGCAATTTATCATGAATTTAAGC
ACGTGAAAGATCATGCTGATTTGATGGCACTATACAACATCCCTATTTTTAGATCAAAAATGGAAGCAGAAGCTGAACAT
TATATGTTCGAATGTTTGATTGAAAAAAACGATGGTCAGTTTAATTATTCTAACGTAATTACACATTATAATTTAAAAAT
GGGGCAAGAAACTTATCTAAAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14106.92 Da
• Isoelectric Point: 4.3486

>AT150002 WP_154635730.1 NZ_CP048796:c848782-848414 [Providencia vermicola]
MDEYSPKNYDISELKYLCNSLNREAISSLQKTNTHWINDLSSPQSVRLNELIEHIAAFVWQYKIKHPKDNLVISLVEEYL
DETYDLFGSPVITLSEIIDWQSMNQNLVAVLDDDLKCPASKT

Nucleotide

• Length: 369 bp

>AT150002 NZ_CP064374:392762-393094 [Enterococcus faecalis]
ATGACAGCATTTGATAGAATAAAAAAAATTACAGACCGTAGAGGTATTTCCATTAATGAGTTAGAAAATCGAGTTGGAAT
TAGTCAAAATGTATTATATGGATGGAAGAAAAAAACTCCTGGCGGTGAAAATTTGACTAAAGTTGCTAAATATTTAAATA
TTTCCACTGATTATATTTTAGGAATAGTTGATAATCCAGAACCTTTTGTCGAAAAAAAAACTGATGATTTAGACGATGTA
CTAGATAACGTCATGAGCTTTGATGGTGAACCACTTGATGATCATGACAGAGAAGTTATTCGTGCTTACTTGAAAGGAAG
ATTCGGAAAATAA

Structures

Toxin

• AlphaFold DB: K8W3H1