Detailed information of TA system TA150001

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1323143..1323751
• Replicon: chromosome
• Accession: NZ_CP048794
• Organism: Paenibacillus polymyxa E681 isolate

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: GE561_RS06020
• Protein ID: WP_007429335.1
• Coordinates: 1323401..1323751 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GE561_RS06015
• Protein ID: WP_170970806.1
• Coordinates: 1323143..1323394 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE561_RS06005	1320087..1321364	+	1278	WP_013309245.1	outer membrane lipoprotein carrier protein LolA	-
GE561_RS06010	1321623..1322819	+	1197	WP_013309246.1	alanine racemase	-
GE561_RS06015	1323143..1323394	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
GE561_RS06020	1323401..1323751	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GE561_RS06025	1323958..1326153	+	2196	WP_190845174.1	RNA-binding transcriptional accessory protein	-
GE561_RS06030	1326258..1326392	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
GE561_RS06035	1326537..1327049	+	513	WP_043882100.1	SprT family protein	-
GE561_RS06050	1327437..1328312	-	876	WP_013309249.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T150001 WP_007429335.1 NZ_CP048794:1323401-1323751 [Paenibacillus polymyxa E681]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T150001 NZ_CP064372:1476-1751 [Klebsiella pneumoniae]
ATGACCACAAGGACGATTGAATACTCAGGCCAGTTCCAGAAAGATGTGAAGAAGGCTCAGAAGCGTCATAAAGACATGAG
CAAGCTTAAAGCCCTTATGACGCTGTTAATCAATGATGCCTTGCCATTGCCTCAAGTCTACAAAGATCACCCTCTACAGG
GGAACTACAAAGGCTATAGGGATGCTCACATAGAACCAGACTGGCTACTCATCTACAAGATAACTGATGAAGTCCTACGC
TTTGAGCGAACCGGAACACATTCAGACCTGTTTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT150001 WP_170970806.1 NZ_CP048794:1323143-1323394 [Paenibacillus polymyxa E681]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT150001 NZ_CP064372:1212-1472 [Klebsiella pneumoniae]
ATGGCCGCAAATGCATTTGTTCGTGCTCGTATAGATGAAACACTGAAAAATGAAGCTGCAGTAGTGCTTGCCGGAATGGG
CTTAACCGTTTCCGATCTGGTTCGTATCACTCTGACTAAAGTAGCCAAAGAAAAGGCTCTGCCGTTCGATATGCGTGTTC
CTAACGAGCTTACTGCTAATACCATCGCTAACAGTGAGAAAGGCGTAGACGTTCATACAGCTAAAGATGCTGATGATCTG
TTTAATCAGCTTGGTATCTGA

Structures

Toxin

• AlphaFold DB: H6CFY1