Detailed information of TA system TA150000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1323161..1323769
• Replicon: chromosome
• Accession: NZ_CP048793
• Organism: Paenibacillus polymyxa E681 isolate

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: GMA19_RS05960
• Protein ID: WP_007429335.1
• Coordinates: 1323419..1323769 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GMA19_RS05955
• Protein ID: WP_170970806.1
• Coordinates: 1323161..1323412 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMA19_RS05945	1320105..1321382	+	1278	WP_013309245.1	outer membrane lipoprotein carrier protein LolA	-
GMA19_RS05950	1321641..1322837	+	1197	WP_013309246.1	alanine racemase	-
GMA19_RS05955	1323161..1323412	+	252	WP_170970806.1	ribbon-helix-helix protein, CopG family	Antitoxin
GMA19_RS05960	1323419..1323769	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GMA19_RS05965	1323976..1326171	+	2196	WP_190845174.1	RNA-binding transcriptional accessory protein	-
GMA19_RS05970	1326276..1326410	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
GMA19_RS05975	1326555..1327067	+	513	WP_043882100.1	SprT family protein	-
GMA19_RS05990	1327455..1328330	-	876	WP_013309249.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-36)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T150000 WP_007429335.1 NZ_CP048793:1323419-1323769 [Paenibacillus polymyxa E681]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T150000 NZ_CP064371:c27049-26633 [Klebsiella pneumoniae]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAACCGTCCTGAAACGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTGACGAATAATACAAGAGAATTTGAGCGGGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9465.86 Da
• Isoelectric Point: 6.4671

>AT150000 WP_170970806.1 NZ_CP048793:1323161-1323412 [Paenibacillus polymyxa E681]
MISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEEDANSTLGRTV
SGV

Nucleotide

• Length: 252 bp

>AT150000 NZ_CP064371:c27276-27046 [Klebsiella pneumoniae]
ATGAGAACCGTATCGATATTTAAAAATGGCAACAACCGCGCCATCCGTCTGCCCCGTGATCTGGATTTTGAGGGGGTGAG
AGAGCTCGAGATCATCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGCTCATTCCTGGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: H6CFY1