Detailed information of TA system TA149998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 831045..831619
• Replicon: chromosome
• Accession: NZ_CP048792
• Organism: Pasteurella multocida strain VP161

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: PmVP161_RS03785
• Protein ID: WP_061406134.1
• Coordinates: 831335..831619 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: PmVP161_RS03780
• Protein ID: WP_135938169.1
• Coordinates: 831045..831338 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PmVP161_RS03760	826204..827295	-	1092	WP_005751571.1	L-ascorbate 6-phosphate lactonase	-
PmVP161_RS03765	827645..829435	+	1791	WP_061406132.1	PTS ascorbate-specific subunit IIBC	-
PmVP161_RS03770	829480..829947	+	468	WP_064661066.1	PTS sugar transporter subunit IIA	-
PmVP161_RS03775	829965..830642	+	678	WP_005726756.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-
PmVP161_RS03780	831045..831338	-	294	WP_135938169.1	putative addiction module antidote protein	Antitoxin
PmVP161_RS03785	831335..831619	-	285	WP_061406134.1	hypothetical protein	Toxin
PmVP161_RS03795	832898..833257	-	360	WP_095261631.1	DUF3418 domain-containing protein	-
PmVP161_RS03800	833317..833952	+	636	Protein_716	NERD domain-containing protein	-
PmVP161_RS03805	834288..835826	+	1539	WP_061406430.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-87)

Antitoxin

• Uncharacterized protein HI1420 (5-92)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10667.40 Da
• Isoelectric Point: 10.2297

>T149998 WP_061406134.1 NZ_CP048792:c831619-831335 [Pasteurella multocida]
VGRLKEVKILSAKSAVLARINKAMNGNFGDHKSIGNGLYEMRIIKGSGYRVYYGQYREVTYLLICGGDKSTQKSDIVKAR
ELWKEIKQQEEVKV

Nucleotide

• Length: 285 bp

>T149998 NZ_CP064370:c97375-96959 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCATTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCAGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAAACCTCCCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCAGTGGACGCCACGACGGGCATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCTATTGC
CGGCCACCCCATCGCGGCCGGCGCCGTCCTGGTGACGAACAATGTGCGGGAGTTTGCGCGGGTCCCGGGTCTGGTGCTGG
AAGACTGGGTGGAATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10948.83 Da
• Isoelectric Point: 7.2766

>AT149998 WP_135938169.1 NZ_CP048792:c831338-831045 [Pasteurella multocida]
MSKKKIKLKLFDIAEHLDSEEMIAAYLSEVLETCDTNEFISALSDVVRAKGMTELAAKTGLRRESLYKTLSYGSEPRFDT
IMKIMHSLGIKLVPTHI

Nucleotide

• Length: 294 bp

>AT149998 NZ_CP064370:c97602-97372 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGAGGGGGTGAG
CGAGCAGGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAACTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGAATGTGGTCAGCGATGAAGGACGCTTTAAGCCATGA