Detailed information of TA system TA149996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 4025411..4026982
• Replicon: chromosome
• Accession: NZ_CP048787
• Organism: Proteus mirabilis strain CC15031

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B4F0T8
• Locus tag: G4D35_RS18735
• Protein ID: WP_012368637.1
• Coordinates: 4025411..4026724 (-)
• Length: 438 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: B4F0T9
• Locus tag: G4D35_RS18740
• Protein ID: WP_004249771.1
• Coordinates: 4026725..4026982 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4D35_RS18715	4020579..4021382	+	804	WP_104836842.1	energy transducer TonB	-
G4D35_RS18720	4021436..4023397	-	1962	WP_172767808.1	hypothetical protein	-
G4D35_RS18725	4023429..4024358	-	930	WP_004246518.1	metallophosphoesterase	-
G4D35_RS18730	4024543..4025007	-	465	WP_012368636.1	DUF1097 domain-containing protein	-
G4D35_RS18735	4025411..4026724	-	1314	WP_012368637.1	type II toxin-antitoxin system HipA family toxin	Toxin
G4D35_RS18740	4026725..4026982	-	258	WP_004249771.1	helix-turn-helix domain-containing protein	Antitoxin
G4D35_RS18745	4027131..4028495	-	1365	WP_012368638.1	tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE	-
G4D35_RS18750	4028633..4030273	-	1641	WP_004246512.1	membrane protein insertase YidC	-
G4D35_RS18755	4030276..4030536	-	261	WP_012368639.1	membrane protein insertion efficiency factor YidD	-
G4D35_RS18760	4030500..4030859	-	360	WP_004246510.1	ribonuclease P protein component	-
G4D35_RS18765	4030877..4031020	-	144	WP_004246509.1	50S ribosomal protein L34	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 438 a.a.
• Molecular weight: 49670.78 Da
• Isoelectric Point: 6.5023

>T149996 WP_012368637.1 NZ_CP048787:c4026724-4025411 [Proteus mirabilis]
MASLDVYMNEYYVGIFTKKSSGAHSFQYTEDWVNQPGSRPISLSMPLQLQEYQGDCVYNFFDNLLPDNTEIRNRIVSRYN
ANSNQPFDLLAAIGADTVGALRLLPSGSTPADIKKIEYQKLNDIELEKILLGYKSNTPLGMLKEYTDFRISIAGAQEKTA
LLLKDNHWCVPHNNTPTTHIIKLPIGVIESHSYTMDLSNSVENEYLCTLIAKEFDLPVPHCSIIKTKNVKALAVERFDRK
YSSDKSWIIRLPQEDFCQILNIPSALKYENEGGPGIIDIMKYLLGSSNADQDRFHFMKAQVLFWLLAATDGHAKNFSVFI
NQEGRFHLTPLYDIMSIYPNFGGRGLNPRDAKLAMGLKASRGKKYAIEQIFPRHFYQTAEAVGFDPSLMSDILRYFYQEM
DDVIQRVKQQLPEDFPSEISNTILDGMKARAVRLANI

Nucleotide

• Length: 1314 bp

>T149996 NZ_CP064369:1201-1467 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGACCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGGATTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCATTCTGTGTGAGATCCGGGATGACGAGCTGGTTATCCTTGCCGCCACCATT
GGCCATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9878.43 Da
• Isoelectric Point: 10.0139

>AT149996 WP_004249771.1 NZ_CP048787:c4026982-4026725 [Proteus mirabilis]
MIIINAYQLSVHLKDVRLTKKLSQSKVAQKVGIRQDTVSNFELNPNSTKLETFFKLLSALNLEMEIHPKDAKNNLAHKSE
WKEEW

Nucleotide

• Length: 258 bp

>AT149996 NZ_CP064369:993-1217 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACCTT
CTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAAGATTATTATCTTTCAGCAGAAACTGCCGCACGCGTTC
GCCGTGGTGATGAAGCCGTGCATTCATCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: B4F0T8

Antitoxin

• AlphaFold DB: B4F0T9