Detailed information of TA system TA149992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3162297..3163318
• Replicon: chromosome
• Accession: NZ_CP048787
• Organism: Proteus mirabilis strain CC15031

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: A0A8E4FRK4
• Locus tag: G4D35_RS14805
• Protein ID: WP_017827532.1
• Coordinates: 3162749..3163318 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: G4D35_RS14800
• Protein ID: WP_004245528.1
• Coordinates: 3162297..3162749 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4D35_RS14780	3158083..3158613	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
G4D35_RS14785	3158882..3159895	+	1014	WP_004250228.1	thiamine ABC transporter substrate binding subunit	-
G4D35_RS14790	3159895..3161475	+	1581	WP_004245526.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
G4D35_RS14795	3161468..3162169	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
G4D35_RS14800	3162297..3162749	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
G4D35_RS14805	3162749..3163318	+	570	WP_017827532.1	PIN domain-containing protein	Toxin
G4D35_RS14810	3163434..3165788	+	2355	WP_036895567.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21601.08 Da
• Isoelectric Point: 7.0180

>T149992 WP_017827532.1 NZ_CP048787:3162749-3163318 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKDPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T149992 NZ_CP064368:c3084291-3083908 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT149992 WP_004245528.1 NZ_CP048787:3162297-3162749 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT149992 NZ_CP064368:c3084533-3084291 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: B4F2H3