Detailed information of TA system TA149977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1085506..1086133
• Replicon: chromosome
• Accession: NZ_CP048784
• Organism: Serratia liquefaciens strain S1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: F0336_RS05030
• Protein ID: WP_197821222.1
• Coordinates: 1085506..1085727 (-)
• Length: 74 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A379ZYL8
• Locus tag: F0336_RS05035
• Protein ID: WP_020825515.1
• Coordinates: 1085765..1086133 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F0336_RS05015	1081145..1082488	-	1344	WP_149573597.1	NCS2 family permease	-
F0336_RS05020	1082571..1084358	-	1788	WP_149573598.1	adenine deaminase	-
F0336_RS05025	1084497..1085429	+	933	WP_020825513.1	LysR family transcriptional regulator	-
F0336_RS05030	1085506..1085727	-	222	WP_197821222.1	hemolysin expression modulator Hha	Toxin
F0336_RS05035	1085765..1086133	-	369	WP_020825515.1	Hha toxicity modulator TomB	Antitoxin
F0336_RS05040	1086296..1086622	-	327	WP_129982675.1	hypothetical protein	-
F0336_RS05045	1086952..1087098	+	147	WP_020825517.1	hypothetical protein	-
F0336_RS05050	1087095..1087817	+	723	WP_046374340.1	ABC transporter ATP-binding protein	-
F0336_RS05055	1087804..1088661	+	858	WP_041414279.1	metal ABC transporter permease	-
F0336_RS05060	1088687..1089565	+	879	WP_149573599.1	metal ABC transporter substrate-binding protein	-
F0336_RS05065	1089678..1089818	-	141	WP_012005563.1	type B 50S ribosomal protein L36	-
F0336_RS05070	1089831..1090085	-	255	WP_020825521.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1077224..1091456	14232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (14-70)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 74 a.a.
• Molecular weight: 8727.21 Da
• Isoelectric Point: 7.9877

>T149977 WP_197821222.1 NZ_CP048784:c1085727-1085506 [Serratia liquefaciens]
ITPKVIMTKTDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Nucleotide

• Length: 222 bp

>T149977 NZ_CP064363:c3742565-3742347 [Salmonella enterica subsp. arizonae]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAACTGTCCGATAATGAGTTAGCTGTATTTTACTCAGCGGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTTTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14189.89 Da
• Isoelectric Point: 4.5140

>AT149977 WP_020825515.1 NZ_CP048784:c1086133-1085765 [Serratia liquefaciens]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSSVNLQLNELIEHIASFVMSYKIKYMDESDLSELVEEYL
DDTYTLFSSYGINDSDLRRWQKTKARLFRMFSGEGICTTMKS

Nucleotide

• Length: 369 bp

>AT149977 NZ_CP064363:c3742967-3742593 [Salmonella enterica subsp. arizonae]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTTGGTTAATGACCCAACCTCAGCAGTCAATCTCCAGCTCAATGAGCTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAGATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTCAATCGGGTAACCGACT
GTTTCGCTGTTTCGTCAACGCCACAAGGGCCAACCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A379ZYL8