Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 91283..91809 Replicon plasmid pEGKP7.2
Accession NZ_CP048780
Organism Klebsiella pneumoniae strain EGKP7

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag G4D19_RS27730 Protein ID WP_000323025.1
Coordinates 91522..91809 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag G4D19_RS27725 Protein ID WP_000534858.1
Coordinates 91283..91522 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4D19_RS27705 (G4D19_28200) 87611..88849 + 1239 WP_003030308.1 IS110 family transposase -
G4D19_RS27710 (G4D19_28205) 89325..89897 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
G4D19_RS27715 (G4D19_28210) 90097..91020 + 924 WP_020277922.1 cation diffusion facilitator family transporter -
G4D19_RS27720 91154..91258 - 105 Protein_113 hypothetical protein -
G4D19_RS27725 (G4D19_28215) 91283..91522 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
G4D19_RS27730 (G4D19_28220) 91522..91809 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
G4D19_RS27735 (G4D19_28225) 91876..92910 - 1035 Protein_116 IS481 family transposase -
G4D19_RS27740 92971..93063 + 93 Protein_117 Hok/Gef family protein -
G4D19_RS27745 93027..93128 + 102 Protein_118 DUF5431 family protein -
G4D19_RS27750 (G4D19_28235) 93293..93778 - 486 WP_020277937.1 hypothetical protein -
G4D19_RS27755 (G4D19_28240) 93968..94240 + 273 WP_023280873.1 hypothetical protein -
G4D19_RS27760 (G4D19_28245) 94237..94587 + 351 WP_020277938.1 hypothetical protein -
G4D19_RS27765 (G4D19_28250) 95104..95484 + 381 WP_015632488.1 hypothetical protein -
G4D19_RS27770 (G4D19_28255) 95550..95897 + 348 WP_020316654.1 hypothetical protein -
G4D19_RS27775 (G4D19_28260) 95985..96215 + 231 WP_020805755.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / aph(3')-VI / qnrS1 / blaSHV-12 / aac(6')-Il / blaOXA-10 / qacE / sul1 - 1..153286 153286


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T149975 WP_000323025.1 NZ_CP048780:91522-91809 [Klebsiella pneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T149975 NZ_CP064363:c1808813-1808481 [Salmonella enterica subsp. arizonae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCGCTGGATCCGATTGCCGGTCACGAGCAAAGCGGAAAACGTCCGGTACTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTGCCCGTTGTCGTTCCCGTCACCAGCGGCGGAAACTTCGCCCGCA
CAGCCGGTTTTGCCGTCTCACTGGACGGCGCGGGAACCAGAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGCGCCCGGAACGGTAAGCGGCTGGAACGTCTACCCGACGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAAGC
TATTCTGAGCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT149975 WP_000534858.1 NZ_CP048780:91283-91522 [Klebsiella pneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT149975 NZ_CP064363:c1809071-1808814 [Salmonella enterica subsp. arizonae]
ATGTATACCACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCTGCTGTGCTGAAAACGCTGGCGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAATGGCTGCCTGATTATTGAACCCCGGAAACGGCCCTGTTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCAAACGCCGAAATAAGCGAAGAGGATCGGGAATGGATTGATGCGCCAGCGGTG
GGCAGGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References