Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 54641..55284 Replicon plasmid pEGKP7.2
Accession NZ_CP048780
Organism Klebsiella pneumoniae strain EGKP7

Toxin (Protein)


Gene name vagD Uniprot ID A0A7D6UII7
Locus tag G4D19_RS27505 Protein ID WP_032425563.1
Coordinates 54641..55057 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag G4D19_RS27510 Protein ID WP_001261276.1
Coordinates 55054..55284 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4D19_RS27490 (G4D19_27960) 49685..51793 - 2109 WP_032427226.1 peptidase domain-containing ABC transporter -
G4D19_RS27495 (G4D19_27965) 51783..53060 - 1278 WP_009309915.1 HlyD family secretion protein -
G4D19_RS27500 (G4D19_27970) 53722..54600 + 879 WP_009309916.1 restriction endonuclease -
G4D19_RS27505 (G4D19_27975) 54641..55057 - 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin Toxin
G4D19_RS27510 (G4D19_27980) 55054..55284 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
G4D19_RS27515 (G4D19_27985) 55337..55711 + 375 WP_171483593.1 hypothetical protein -
G4D19_RS27520 (G4D19_27995) 55872..56816 + 945 WP_011977810.1 hypothetical protein -
G4D19_RS27525 (G4D19_28000) 56853..57245 + 393 WP_011977811.1 hypothetical protein -
G4D19_RS27530 (G4D19_28005) 57303..57824 + 522 WP_013214008.1 hypothetical protein -
G4D19_RS27535 (G4D19_28010) 57870..58073 + 204 WP_011977813.1 hypothetical protein -
G4D19_RS27540 (G4D19_28015) 58103..59107 + 1005 WP_011977814.1 hypothetical protein -
G4D19_RS27545 (G4D19_28025) 59291..60070 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / aph(3')-VI / qnrS1 / blaSHV-12 / aac(6')-Il / blaOXA-10 / qacE / sul1 - 1..153286 153286


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15080.56 Da        Isoelectric Point: 8.5403

>T149974 WP_032425563.1 NZ_CP048780:c55057-54641 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T149974 NZ_CP064363:c1731927-1731520 [Salmonella enterica subsp. arizonae]
GTGGTCCAGTGGCAATCTGATTTACGCGTCTCATGGCGTGCTCAGTGGATCTCATTACTCATTCACGGGTTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTGTGGATGATGCTACTGTCGCTGGTCGTGTTTGACTGCG
TACGTAGCCAGCGCCGGATTAATACCTGTCAGGGAGAGATCAAGCTGCTGATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCCTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCTGAGTCTGGACGCCATCA
GCATTTATGGCTGGCGGCTGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATAATGTTACAGCAACCGATAT
CGCGCTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT149974 WP_001261276.1 NZ_CP048780:c55284-55054 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT149974 NZ_CP064363:c1732174-1731908 [Salmonella enterica subsp. arizonae]
ATGGATATTAACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
CTTTGAACATGAGTACGATAGTTTGAGCGACGGAGAAAAGCGTATTTTTATCCGCTTGTTGCAGAATGATGACCCAGATT
TATTTAACTGGTTGATGAATCACGGTAAGCCGGCTGACGCTGAGCTGGAACAGATGGTACGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCAGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6UII7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References