Detailed information of TA system TA149971

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5252799..5253424
• Replicon: chromosome
• Accession: NZ_CP048778
• Organism: Klebsiella pneumoniae strain EGKP7

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: G4D19_RS25745
• Protein ID: WP_238952526.1
• Coordinates: 5252799..5253182 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: G4D19_RS25750
• Protein ID: WP_004150355.1
• Coordinates: 5253182..5253424 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4D19_RS25730 (5250165)	5250165..5251067	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
G4D19_RS25735 (5251064)	5251064..5251699	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
G4D19_RS25740 (5251696)	5251696..5252625	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
G4D19_RS25745 (5252799)	5252799..5253182	-	384	WP_238952526.1	type II toxin-antitoxin system VapC family toxin	Toxin
G4D19_RS25750 (5253182)	5253182..5253424	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
G4D19_RS25755 (5253629)	5253629..5254546	+	918	WP_023302328.1	alpha/beta hydrolase	-
G4D19_RS25760 (5254560)	5254560..5255501	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
G4D19_RS25765 (5255546)	5255546..5255983	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
G4D19_RS25770 (5255980)	5255980..5256840	-	861	WP_004146232.1	virulence factor BrkB family protein	-
G4D19_RS25775 (5256834)	5256834..5257433	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14407.64 Da
• Isoelectric Point: 7.3178

>T149971 WP_238952526.1 NZ_CP048778:c5253182-5252799 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDTIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T149971 NZ_CP064363:719030-719362 [Salmonella enterica subsp. arizonae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCGCTGGATCCGATTGCCGGCCACGAGCAAAGCGGAAAACGTCCGGTACTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGCTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAAACTTCGCCCGCA
CAGCCGGTTTTGCCGTCTCACTGGACGGCGCGGGAACCAGAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGCGCCCGGAACGGTAAGCGGCTGGAACGTCTACCCGACGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT149971 WP_004150355.1 NZ_CP048778:c5253424-5253182 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT149971 NZ_CP064363:718772-719029 [Salmonella enterica subsp. arizonae]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCGTCATGCTAGCGGTTCCTCCTGCTGTGCTGAAAACGCTGGCGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAATGGCTGCCTGATTATTGAACCCCGGAAACGGCCCTGCTATTCGC
TTGAGGAACTGCTGGCGCAGTGCGATCCAAACGCCGAAATAAGCGAAGAGGATCGGGAATGGATTGATGCGCCAGCGGTG
GGCAGGGAGATCCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9