Detailed information of TA system TA149970

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4934420..4936101
• Replicon: chromosome
• Accession: NZ_CP048778
• Organism: Klebsiella pneumoniae strain EGKP7

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: J2XCI1
• Locus tag: G4D19_RS24290
• Protein ID: WP_004146663.1
• Coordinates: 4934420..4935337 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A6TH05
• Locus tag: G4D19_RS24295
• Protein ID: WP_004177809.1
• Coordinates: 4935343..4936101 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4D19_RS24270 (4929726)	4929726..4931246	+	1521	WP_004210021.1	sugar ABC transporter ATP-binding protein	-
G4D19_RS24275 (4931243)	4931243..4932241	+	999	WP_012737175.1	ribose ABC transporter permease	-
G4D19_RS24280 (4932272)	4932272..4933213	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
G4D19_RS24285 (4933288)	4933288..4934295	+	1008	WP_004178300.1	carbohydrate kinase family protein	-
G4D19_RS24290 (4934420)	4934420..4935337	-	918	WP_004146663.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
G4D19_RS24295 (4935343)	4935343..4936101	-	759	WP_004177809.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
G4D19_RS24300 (4936187)	4936187..4936915	-	729	WP_002885152.1	response regulator transcription factor	-
G4D19_RS24305 (4937188)	4937188..4939335	+	2148	WP_238952516.1	formate dehydrogenase subunit alpha	-
G4D19_RS24310 (4939565)	4939565..4940251	+	687	WP_004151733.1	tetratricopeptide repeat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (1-93)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34659.10 Da
• Isoelectric Point: 5.4707

>T149970 WP_004146663.1 NZ_CP048778:c4935337-4934420 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIAYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T149970 NZ_CP064363:c565451-565348 [Salmonella enterica subsp. arizonae]
GGCAAGGCGACGTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28548.92 Da
• Isoelectric Point: 10.0870

>AT149970 WP_004177809.1 NZ_CP048778:c4936101-4935343 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLAFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT149970 NZ_CP064363:565348-565489 [Salmonella enterica subsp. arizonae]
AAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCAT
TAATGCAGGCTACGTCGCCTTGCCCTTTAAGAATAGATGACAACGCCAGGTTTTCCAGCTTG

Structures

Toxin

• AlphaFold DB: J2XCI1

Antitoxin

• AlphaFold DB: A6TH05