Detailed information of TA system TA149968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4669769..4670579
• Replicon: chromosome
• Accession: NZ_CP048778
• Organism: Klebsiella pneumoniae strain EGKP7

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A332NV98
• Locus tag: G4D19_RS23005
• Protein ID: WP_014908042.1
• Coordinates: 4669769..4670302 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: G4D19_RS23010
• Protein ID: WP_023343051.1
• Coordinates: 4670313..4670579 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4D19_RS23000 (4668600)	4668600..4669721	+	1122	WP_012737335.1	cupin domain-containing protein	-
G4D19_RS23005 (4669769)	4669769..4670302	-	534	WP_014908042.1	type II toxin-antitoxin system toxin KacT	Toxin
G4D19_RS23010 (4670313)	4670313..4670579	-	267	WP_023343051.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
G4D19_RS23015 (4670682)	4670682..4672115	-	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
G4D19_RS23020 (4672105)	4672105..4672788	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
G4D19_RS23025 (4672961)	4672961..4674346	+	1386	WP_024623207.1	efflux transporter outer membrane subunit	-
G4D19_RS23030 (4674364)	4674364..4674708	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19795.68 Da
• Isoelectric Point: 5.2614

>T149968 WP_014908042.1 NZ_CP048778:c4670302-4669769 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T149968 NZ_CP064359:c1722787-1722578 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT149968 WP_023343051.1 NZ_CP048778:c4670579-4670313 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT149968 NZ_CP064359:c1723087-1722794 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Toxin

• AlphaFold DB: A0A332NV98