Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4062363..4062982 Replicon chromosome
Accession NZ_CP048778
Organism Klebsiella pneumoniae strain EGKP7

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag G4D19_RS20090 Protein ID WP_002892050.1
Coordinates 4062764..4062982 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag G4D19_RS20085 Protein ID WP_002892066.1
Coordinates 4062363..4062737 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4D19_RS20075 (4057515) 4057515..4058708 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
G4D19_RS20080 (4058731) 4058731..4061877 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
G4D19_RS20085 (4062363) 4062363..4062737 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
G4D19_RS20090 (4062764) 4062764..4062982 + 219 WP_002892050.1 HHA domain-containing protein Toxin
G4D19_RS20095 (4063141) 4063141..4063707 + 567 WP_002892030.1 maltose O-acetyltransferase -
G4D19_RS20100 (4063679) 4063679..4063819 - 141 WP_004147370.1 hypothetical protein -
G4D19_RS20105 (4063840) 4063840..4064310 + 471 WP_002892026.1 YlaC family protein -
G4D19_RS20110 (4064285) 4064285..4065736 - 1452 WP_023302231.1 PLP-dependent aminotransferase family protein -
G4D19_RS20115 (4065837) 4065837..4066535 + 699 WP_002892021.1 GNAT family protein -
G4D19_RS20120 (4066532) 4066532..4066672 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
G4D19_RS20125 (4066672) 4066672..4066935 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T149967 WP_002892050.1 NZ_CP048778:4062764-4062982 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T149967 NZ_CP064359:c1711735-1711373 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT149967 WP_002892066.1 NZ_CP048778:4062363-4062737 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT149967 NZ_CP064359:c1711902-1711732 [Staphylococcus epidermidis]
ATGTTATCTTTTAATCAAAATAGAAACCACAGTCTTGAACAATCTTTAAAAGAAGGTTATGCACAAATGGCCGATTTAAA
CCTCTCCCTAGCAACAGAAGCTTTCCCGATAGAGTGTGAAGCTTGTGATTGCAATGAATCACATTTAATATCTAATTCAA
AGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References