Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-PrlF
Location 2542943..2543480 Replicon chromosome
Accession NZ_CP048751
Organism Brevundimonas mediterranea strain D151-2-6

Toxin (Protein)


Gene name mazF Uniprot ID B4WB32
Locus tag GYM46_RS12420 Protein ID WP_008264285.1
Coordinates 2542943..2543278 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A069IU01
Locus tag GYM46_RS12425 Protein ID WP_035305435.1
Coordinates 2543265..2543480 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GYM46_RS12400 2538335..2538475 + 141 WP_154725362.1 hypothetical protein -
GYM46_RS12405 2538479..2540167 - 1689 WP_197019690.1 lytic transglycosylase domain-containing protein -
GYM46_RS12410 2540275..2541072 - 798 WP_008261821.1 uracil-DNA glycosylase -
GYM46_RS12415 2541268..2542923 + 1656 WP_156796558.1 electron transfer flavoprotein-ubiquinone oxidoreductase -
GYM46_RS12420 2542943..2543278 - 336 WP_008264285.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GYM46_RS12425 2543265..2543480 - 216 WP_035305435.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
GYM46_RS12430 2543581..2545482 + 1902 WP_164952704.1 hypothetical protein -
GYM46_RS12435 2545488..2546360 + 873 WP_008260339.1 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase -
GYM46_RS12440 2546446..2547339 + 894 WP_008259607.1 glycine--tRNA ligase subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-110)

Antitoxin

PrlF antitoxin

(4-53)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12353.09 Da        Isoelectric Point: 5.8752

>T149953 WP_008264285.1 NZ_CP048751:c2543278-2542943 [Brevundimonas mediterranea]
MPDFERGDVVRIPFPYTDRNTRQHRPGLVVSAGGIGDQAGLIWVVMITSAANRRWPDDLDLEDRYGEAGLPAPSVVRPSK
IATIEASAAERIGRLDEARLAVVMETIRRWL

Download         Length: 336 bp

>T149953 NZ_CP064352:4448116-4448484 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7969.02 Da        Isoelectric Point: 4.7719

>AT149953 WP_035305435.1 NZ_CP048751:c2543480-2543265 [Brevundimonas mediterranea]
MITSRLTSKAQTTIPQPVRAALKLQDGDELIYEIEGERVLLKKARKTAADDPFATFEEWDSEADRKAYAGL

Download         Length: 216 bp

>AT149953 NZ_CP064352:4447898-4448119 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB B4WB32

Antitoxin

Source ID Structure
AlphaFold DB A0A069IU01

References